You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
PSA™ iFluor® 350 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT BIOQUEST
iFluor® 350 PSA kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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PSA™ iFluor® 488 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT BIOQUEST
iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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Buccutite™ Poly-HRP Antibody Conjugation Kit
Supplier: AAT BIOQUEST
Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level.
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ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labelling Kit
Supplier: AAT BIOQUEST
Microscale optimised for labeling 50 µg antibody per reaction. ReadiLink™ Rapid mFluor™ Violet 610 antibody labelling kits provide a quick and convenient method to label antibodies, proteins (<10 kDa), or other amine-containing biomolecules.
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iFluor® 680-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT BIOQUEST
iFluor® 680 WGA conjugate, like Alexa Fluor® 680 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.
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Crosslink immunoprecipitation (IP) kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.
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ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.
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ReadiLink™ Rapid XFD488 Antibody Labelling Kit 'XFD488 Same Structure to Alexa Fluor™ 488' (2×50 µg Labellings)
Supplier: AAT BIOQUEST
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).
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Antibody labelling kits, DyLight™
Supplier: Thermo Fisher Scientific
The DyLight™ Antibody Labeling Kits were specifically developed for fast, efficient labeling of antibodies. Two convenient kit formats are available to accommodate varied labeling requirements. The DyLight™ Antibody Labeling Kits contain all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins.
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HIS Lite™ iFluor® 568 Tris NTA Chelator
Supplier: AAT BIOQUEST
iFluor® 568 Tris-NTA Chelator is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion (such as Ni²⁺) in cells, solution and solid surfaces.
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PSA™ iFluor® 594 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT BIOQUEST
iFluor® 594 PSA kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional
Supplier: Thermo Fisher Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.
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Amine reactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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30 Minute IHC DAB Detection System
Supplier: ANTIBODIES.COM
This 30 Minute IHC DAB Detection System is a powerful intensification system that significantly enhances chromogenic signals. This system can be integrated into standard immunohistochemistry (IHC) staining methods and results in a significant increase in sensitivity compared to standard IHC detection methods, while maintaining similar specificity.
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Dye labeling kit (FITC), HOOK™
Supplier: G-Biosciences
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494 nm and 520 nm, respectively, therefore emitting green visible light.
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ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)
Supplier: AAT BIOQUEST
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).
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CytoWatch™ Wash-free Fluorescence Cell Imaging Buffer, 10X
Supplier: AAT BIOQUEST
CytoWatch™ Wash-free fluorescence cell imaging buffer *10X* is a ready-to-use buffer optimised for fluorescence cell imaging.
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Carboxyl reactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.
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ReadiLink™ iFluor® 488 Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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PSA™ iFluor® 594 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 594 PSA kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT BIOQUEST
iFluor® 532 WGA conjugate, like Alexa Fluor® 532 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.
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Speciality dyes, DyLight™
Supplier: Thermo Fisher Scientific
The DyLight™ Specialty Dyes are fluorescent dyes with varying spectral characteristics, degrees of sulfonation, charges and hydrophobicities. These dyes are offered in their amine-reactive forms and are mainly derivatized with N-hydroxysuccinimide (NHS) esters, the simplest and most commonly used reactive group for labeling proteins. The large selection of near infrared (NIR) and infrared (IR) dyes may be used for in vivo and near-IR fluorescence (NIRF) applications.
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MS-Compatible magnetic IP kit (Protein A/G), Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.
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Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers
Supplier: Thermo Fisher Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.
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Photoreactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
Photoactivatable Biotin, Biotin-LC-ASA , Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups. Psoralen-PEG3-Biotin contains a reactive psoralen group and is
designed to efficiently label nucleic acids. The psoralen group intercalates into the helix of DNA, allowing it to label efficiently and selectively. The psoralen can also stack along with the bases of single-stranded DNA or RNA increasing labeling efficiency and selectivity.
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PSA™ iFluor® 488 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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PSA™ iFluor® 555 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 555 PSA kit is a much superior replacement for Alexa Fluor 555 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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PSA™ iFluor® 647 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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Amine reactive biotinylation kits, EZ-Link™
Supplier: Thermo Fisher Scientific
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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NHS-Azide and NHS-phosphine reagents, Pierce™
Supplier: Thermo Fisher Scientific
The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.