You searched for: Protein Linking and Labeling Systems

Supplier: AAT BIOQUEST

Cy3-Tris NTA compound is a sensitive fluorescent chelator for detecting polyhistidine-labelled proteins in cells, solution, and solid surfaces with the addition of certain metal ions (such as Ni²⁺, Co₂⁺ and more) as a mediator.

Supplier: AAT BIOQUEST

Cy5 Bis NTA-Ni Chelator can be applied either to an SDS-PAGE gel or Western blot membrane for fluorescence imaging or used in living cells in combination with the addition of a heavy metal ion such as Ni²⁺.

Supplier: AAT BIOQUEST

CytoWatch™ Wash-free fluorescence cell imaging buffer *10X* is a ready-to-use buffer optimised for fluorescence cell imaging.

Supplier: Thermo Fisher Scientific

ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Supplier: AAT BIOQUEST

XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).

Supplier: AAT BIOQUEST

iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT BIOQUEST

iFluor® 555 PSA kit is a much superior replacement for Alexa Fluor 555 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT BIOQUEST

iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: Thermo Fisher Scientific

A common target for protein modification is the carbohydrate portion of glycoproteins, which can be reacted with hydrazide derivatives of biotin. Oxidative treatment of glycoproteins using 10 mM periodate is used to generate reactive aldehydes from the cis-diols of a variety of carbohydrate moieties.

Supplier: AAT BIOQUEST

Microscale optimised for labelling 25 µg antibody per reaction. APC-iFluor® 750 tandem is an excellent replacement for APC-Cy7 and APC-Alexa Fluor® 750 tandem since they have almost identical spectra.

Supplier: Thermo Fisher Scientific

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.

Supplier: Thermo Fisher Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: AAT BIOQUEST

Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

Supplier: AAT BIOQUEST

iFluor® 647 Tris-NTA Chelator is used as a sensitive red fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion such as Ni²⁺ in cells, solution and solid surfaces.

Supplier: Thermo Fisher Scientific

The DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes. These dyes can be efficiently paired with many DyLight™ dyes and other common fluorescent dyes including fluorescein, rhodamine, cyanine and Texas Red® dye.

Supplier: Thermo Fisher Scientific

Pierce™ Avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates. It is a purified protein from egg whites and it is stable over wide range of pH and temperature.

Supplier: AAT BIOQUEST

iFluor® 532 WGA conjugate, like Alexa Fluor® 532 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

Supplier: G-Biosciences

The HOOK™ Dye Labeling Kit (TAMRA-SE (5-(and-6)- Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labelling of peptides, proteins, nucleic acids and nucleotides. It absorbs green visible light at 546 nm and emits an orange-red visible light at a maximum emission of 575 nm.

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearised plasmid DNA.

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

Supplier: AAT BIOQUEST

iFluor® 594 PSA kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: Thermo Fisher Scientific

Photoactivatable Biotin, Biotin-LC-ASA , Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups. Psoralen-PEG3-Biotin contains a reactive psoralen group and is
designed to efficiently label nucleic acids. The psoralen group intercalates into the helix of DNA, allowing it to label efficiently and selectively. The psoralen can also stack along with the bases of single-stranded DNA or RNA increasing labeling efficiency and selectivity.

Supplier: AAT BIOQUEST

iFluor® 594 PSA kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT BIOQUEST

iFluor® 350 WGA conjugate, like Alexa Fluor® 350 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

Supplier: G-Biosciences

Photoreactive agents, on exposure to ultraviolet light, become active and bind non specifically with neighbouring molecules. These reagents are suitable for labelling molecules that do not contain easily reactable functional groups. Photoreactive reagent HOOK™ Psoralen-PEO-Biotin reacts with and labels nucleic acids and protein molecules. When reacted with nucleic acids, it crosslinks with pyrimidine bases. Crosslinking does not interfere with hybridisation applications.

Supplier: Thermo Fisher Scientific

The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

Supplier: Thermo Fisher Scientific

DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.

Supplier: AAT BIOQUEST

iFluor® 350 PSA kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.