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Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Maleimide-Alkaline Phosphatase, Abcam

Supplier: Abcam

Maleimide-Alkaline Phosphatase. Achieve your results faster with highly validated, pure and trusted compounds.

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Cy3® Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Cy3® conjugation / labeling in < 20 mins with 30 secs hands-on time using Cy3® Conjugation Kit (Fast) - Lightning-Link® ab188287. 100% antibody recovery.

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VeriBlot for IP Detection Reagent (HRP)

Supplier: Abcam

VeriBlot for IP detection reagent (HRP) is an immunoblotting reagent that enables the trouble-free detection of immunoblotted target proteins without interference from denatured IgG. - Cited in over 80 publications - Detects target proteins without interference from IgG heavy and light chains - Recognizes non-reduced antibodies, minimizing detection of denatured IgG - Multiple species compatibility – detects IgG from bovine, goat, human, mouse, rat, rabbit, and sheep (specific isotypes detailed) - Suitable for up to 20 blots with proper dilution (1:200 in 5 ml milk)

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DoubleStain IHC Kit: R&Rt on human/mouse tissue (Green/HRP & AP/Red), Abcam

Supplier: Abcam

Abcam's Doublestain IHC Kit (ab183285) is designed for use with user supplied rabbit and rat primary antibodies to detect two distinct antigens on human and mouse tissue or cell samples.

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rProtein A/G Beads 4FF, Abcam

Supplier: Abcam

Supplemental Information * Ligand: Recombinant Protein A/G * Basic Beads: 4% Cross-linked agarose beads * Particle size: 45μm – 165μm * Storage Buffer: 80% PBS, 20% ethanol * pH Stability Range: 3-10 * Maximum pressure: 0.3 MPa * Binding Capacity: 10-15 mg Rabbit lgG/ml .

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DyLight® 650 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

DyLight® 650 conjugation / labeling in < 20 mins with 30 secs hands-on time using DyLight® 650 Conjugation Kit (Fast) - Lightning-Link® ab201803. 100% antibody recovery.

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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates

Supplier: Biotium

CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.

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Fixable Viability Dye (787/808), Abcam

Fixable Viability Dye (787/808), Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using Fixable Viability Dye (787/808) ab314683. 100% antibody recovery.

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Fluorescein-12-ddUTP

Fluorescein-12-ddUTP

Supplier: Enzo Life Sciences

Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E.coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labelled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.

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R-Phycoerythrin (RPE)

R-Phycoerythrin (RPE)

Supplier: Columbia Biosciences

Many applications and instruments were developed specifically for R-phycoerythrin.

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Tyramide amplification buffer plus

Tyramide amplification buffer plus

Supplier: Biotium

An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

The Abcam PE/R-Phycoerythrin Conjugation Kit (ab102918) offers several standout features: -Rapid Conjugation: achieve PE/R-Phycoerythrin labeling in under 4 hours with just 30 seconds of hands-on time. -High Efficiency: ensures 100% antibody recovery, preserving the integrity and value of your antibodies. -Versatility: suitable for conjugating antibodies, proteins, and peptides, and the labeled antibodies can be used immediately in applications such as Western Blotting (WB), ELISA, and Immunohistochemistry (IHC) without further purification. - Confidence: cited in over 50 publications

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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Alexa Fluor® 488 protein labeling/conjugation kit, Lightning-Link® requires < 20 minutes with 30 seconds hands-on time. - Cited in over 30 publications - 100% antibody recovery - 15-minute incubation time Custom-size conjugation kits up to 100 mg are available on demand.

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Mouse Specific HRP/AEC Detection IHC kit, Abcam

Supplier: Abcam

Mouse specific HRP/AEC IHC Detection Kit ab64258 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.

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Bio-16-ddUTP, tetralithium salt

Bio-16-ddUTP, tetralithium salt

Supplier: Enzo Life Sciences

Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labelled oligonucleotides produced by this reaction are suitable as hybridisation probes and nucleic acid array assays. It is also a substrate for Taq DNA polymerase and other DNA polymerases.

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Gold 525 dUTP (lyophilised)

Supplier: Enzo Life Sciences

Gold 525 dUTP (lyophilised)

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