Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Buffer, TE solution 100x concentrate (TRIS-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)

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Dulbecco’s phosphate buffered saline (DPBS), Sigma-Aldrich®

Supplier: Merck

Dulbecco’s phosphate buffered saline is modified with/without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture.

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pH Buffer solutions, CX-1

Supplier: Thermo Fisher Scientific

Delivers robust reproducible gradients with the Thermo Scientific™ pH buffer platform that is applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, TRIS solution pH 7,6 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7,6 (1 mol/l)

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Wash Solution Concentrate, 20X

Supplier: SERACARE LIFE SCIENCES

Convenient 20X concentrate containing imidazole-buffered saline and Tween® 20.

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Buffer solutions

Supplier: Hanna

Solutions in sachets ensure optimum precision. The plastic-coated sachet provides a perfect liquid seal and good protection from light. The solutions should only be used once to avoid any danger of contamination. They also satisfy the requirements of the GLP and ISO standards. They have been adjusted using a pH meter that has been calibrated according to the NIST standards.

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Buffer, TRIS-tricine-SDS solution 10X concentrate, Sigma-Aldrich®

Supplier: Merck

Running Buffer, TRIS-tricine-SDS buffer solution 10X concentrate

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Phosphate Buffered Saline with 0,05% TWEEN® 20, pH 7,4

Supplier: Merck

Phosphate buffered saline with 0,05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

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Technical pH buffer solutions in sachets, HI 50000 series

Supplier: Hanna

These technical calibration buffers produced in accordance with ISO 3696/BS3978 standards using high purity salts, deionised water, certified weight-checked balances, and Class A glassware in a temperature controlled environment monitored with certified thermometers.

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pH buffer solutions

Supplier: Mettler - Toledo

Technical buffer solution, 6×250 ml, pH value: 4,01

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Buffer, TRIS solution pH 8,0 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 8,0 (1 mol/l)

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pH buffer solutions, certified (DKD)

Supplier: Mettler - Toledo

DKD certified buffer solution, pH value: 10,00, 6× 250 ml

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Buffer solutions in sachets

Supplier: Hanna

For calibration of pH meters.

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Phosphate buffered saline, 10X, for Western blots and IP, Sigma-aldrich®

Supplier: Merck

Phosphate buffered saline is routinely used as a wash buffer in Western blot and immunoprecipitation procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.