Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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J.T.Baker®, Sheath fluid, 3200/3400

Supplier: Avantor

Sheath fluid, 3200/4000, J.T.Baker®

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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology

Supplier: VWR Chemicals

Sodium acetate buffer, VWR®, 3 M, pH value: 5,2

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VWR® Buffer Standard Solutions

Supplier: VWR Chemicals

Buffer Standard solution, Biotechnology Grade, pH value: 4,00, ±0,03, Reference temperature: 25 °C, VWR®, Bottle

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VWR® Buffer, MES-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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VWR® AVS TITRINORM Buffer Solution, pH 9 (at 20 °C), Borate free

Supplier: VWR Chemicals

Solution prepared from AnalaR® NORMAPUR® grade analytical reagents.

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VWR®, Phosphate Buffered Saline (PBS), pH 7,4

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.

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VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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J.T.Baker®, Sheath fluid, 3000/3500

Supplier: Avantor

Sheath fluid 3000/3500, J.T.Baker®

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VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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VWR®, Buffers, Sodium acetate

Supplier: VWR Chemicals

Sodium acetate buffer C, VWR®

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VWR® Buffer, Ammonium Chloride, pH 10, Reag. Ph.Eur.

Supplier: VWR Chemicals

The reagent is specially intended for use in testing Ph Eur and monographs. This solution was produced according 4.1.1 of Ph Eur specifications using Ph Eur reagent quality components and water meeting the Ph Eur monograph requirement for purified water.

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J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

Supplier: Avantor

DILUT-IT is a ready-to-use dissolution media concentrate, which is pre-measured and only need to be diluted with purified water.

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VWR®RapidBlock™, Blocking solution, 10x

Supplier: VWR Chemicals

RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.