Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Buffer solution pH 5, Acetate Buffer

Supplier: Thermo Fisher Scientific

Acetate buffer, Contains 0.7 ml glacial acetic acid and 0.6 g potassium hydroxide per litre., pH value: 4,98 - 5,02 (20 °C)

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HeLa Nuclear Extract, Abcam

Supplier: Abcam

Nuclear extract is prepared from HeLa cells, which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.

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TE Buffer (1X) pH 8,0 for Molecular Biology

Supplier: Apollo Scientific

Cloning Buffer, TE buffer (1X)

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Buffer, PBS tablets

Supplier: PanReac AppliChem

PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.

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SIRT assay buffer

Supplier: Enzo Life Sciences

SIRT assay buffer

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4

Supplier: Cayman Chemical

Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).

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PBS and TBS buffers

Supplier: Rockland Immunochemicals

Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

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Caspase assay buffer

Supplier: Enzo Life Sciences

50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.

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Kinase Dilution Buffer III, Abcam

Supplier: Abcam

Solution of buffer used for diluting kinase protein.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Thermo Fisher Scientific

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Blocking buffer, fish gelatin

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

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Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)

Supplier: Rockland Immunochemicals

Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.

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TPE buffer, 10X tris-phosphate-EDTA, pH 8

Supplier: Rockland Immunochemicals

Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)

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In situ hybridisation wash reagent

Supplier: Enzo Life Sciences

Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.

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Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.