Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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VWR®, Phosphate Buffered Saline (PBS), pH 7,4

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.

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VWR® AVS TITRINORM Buffer Solution, pH 9 (at 20 °C), Borate free

Supplier: VWR Chemicals

Solution prepared from AnalaR® NORMAPUR® grade analytical reagents.

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VWR®, Buffers, Sodium acetate

Supplier: VWR Chemicals

Sodium acetate buffer A, VWR®

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J.T.Baker®, Sheath fluid, 3000/3500

Supplier: Avantor

Sheath fluid 3000/3500, J.T.Baker®

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VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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VWR®, pH electrode maintenance kit

Supplier: VWR Chemicals

The measurement of the pH value is one of the most frequently and universally made measurements in science.

What is often not fully understood is the fact that a large number of problems related to pH measurement is the
correct selection, care or maintenance of the electrodes with particular emphasis on the reference electrode.

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J.T.Baker® BAKER ANALYZED®, Buffer Solutions

Supplier: Avantor

Ready-to-use pH buffers are manufactured in large lots to stringent specifications and utilise quality control procedures to reduce lot-to-lot variability. You can eliminate the time and expense of preparation and standardisation of solutions, helping streamline high-volume analytical work in applications such as quality control.