Assays

Supplier: VWR Chemicals

The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.

Supplier: VWR Chemicals

Fluorescent DNA Quantitation Kit uses fluorescent bisBenzimide H33258 dye to bind to AT sequences of the minor groove of double-stranded DNA. In the assay, the sample is excited at 360 nm and when the dye binds to dsDNA an emission spectrum at 460 nm is produced.

Supplier: Merck

The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Supplier: Apollo Scientific

Test kit for the high sensitive detection of Gaussia Luciferase in mammalian cells or similar applications. The kit includes a reaction buffer, a lysis-reagent suitable for the common mammalian cells, the Substrate and a reconstruction buffer. This Kit is properly for the flash detection of the Luciferase, means that the most sensitive light signal will be perfectly detectable from 5 to 25 sec.

Supplier: Apollo Scientific

Test kit for the Glow-type detection of Gaussia Luciferase in mammalian cells or similar applications. The kit includes a reaction buffer, a lysis-reagent suitable for the common mammalian cells, the Substrate and a reconstruction buffer. This Kit is properly for the Glow detection of the Luciferase, means that the linear light signal will be perfectly detectable from 5 to 25 min.

Supplier: Apollo Scientific

Protease inhibitor cocktail tablets are supplied in a glass vial and each tablet is sufficient for a 50 ml extraction solution.

Supplier: Apollo Scientific

Test kit for the high sensitive detection of Renilla Luciferase in mammalian cells or similar applications. The kit includes a reaction buffer, a lysis-reagent suitable for the common mammalian cells, the Substrate and a reconstruction buffer.

Supplier: Apollo Scientific

Test kit for the detection of Firefly (Photinus Pyralis) Luciferase in cells or similar applications. The kit includes beside the reaction buffer, the substrate and the Cofactor, a Lysis-reagent for the lysis of the common mammalian cells. The Luciferase can be linear detected from 5 sec to 25 min

Supplier: Cayman Chemical

Cayman’s LipidLaunch™ LNP Apparent pK_a Assay Kit (TNS Method) provides a robust and easy-to-use platform for testing the apparent/global pK_a of formulated LNPs.

Supplier: MEGAZYME

The Protein Digestibility Assay Kit (K-PDCAAS) is used for in vitro measurement of the Animal-Safe Accurate Protein Quality Score (ASAP-Quality Score Method).

Supplier: Aviva Systems Biology

S100A11 ELISA kit (Human) is based on standard sandwich enzyme linked immuno-sorbent assay technology. An antibody specific for S100A11 has been pre-coated onto a 96 wellplate (12×8 well strips).

Supplier: Novus Biologicals

Total Cholesterol Assay Kit

Supplier: Novus Biologicals

p62/SQSTM1 (ubiquitin-binding protein p62/Sequestrosome-1) is an intracellular protein (theoretical molecular weight 47,7 kDa) which localises to the cytoplasm, nucleus, autophagosomes and lysosomes in all tissues.

Supplier: Cayman Chemical

Cayman’s LipidLaunch™ BODIPY SM-102 LNP kit (loadable) provides cargo-ready, empty, BODIPY-labelled SM-102 LNPs. LNPs are capable of rapid encapsulation of nucleic acids, without the need for incubation steps, enabling subsequent delivery to target cells or other downstream research applications. Also demonstrate very low toxicity compared to traditional lipid-based transfection methods.

Supplier: Cayman Chemical

Cayman’s LipidLaunch™ LNP-0315 uptake kit (Green Fluorescence) is intended to serve as a starting point for laboratories to explore the uptake of LNPs by their target cell type without the need for specialised equipment. Optimal preparation conditions for the encapsulation of nucleic acids with LNPs must be determined by the end user.