You searched for: Running Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

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IPG buffers

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.

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DIGE buffer kit

Supplier: Cytiva

DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.

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DALT Buffer kit for precast gels

Supplier: Cytiva

DALT Buffer kit is used together with DALT Gel 12,5, which comprises concentrated buffers for running the gel, gel buffer for inserting the gel into DALT precast gel cassette and sealing solution for application of the IPG strip. The buffer in the gel is part of a buffer system that gives longer shelf-life.

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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, TRIS-tricine-SDS solution 10X concentrate, Sigma-Aldrich®

Supplier: Merck

Running Buffer, TRIS-tricine-SDS buffer solution 10X concentrate

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).