You searched for: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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Polyadenylic acid (poly-A)

Supplier: Cytiva

Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.

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Expression vectors, cypridina Luc vector for luciferase assays, Pierce™

Supplier: Thermo Fisher Scientific

Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca.

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Vector, pUC 18, MP Biomedicals

Supplier: MP Biomedicals

pUC 18 is a small vector of approximately 2,7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.

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Expression vectors, red firefly Luc vector for Luciferase assays, Pierce™

Supplier: Thermo Fisher Scientific

Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.

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Gaussia Luc vector for luciferase assays, expression vectors, Pierce™

Supplier: Thermo Fisher Scientific

Gaussia Luc vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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PUC18 plasmid DNA

Supplier: Merck

Plasmids are isolated from host strain E. coli RRI. These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains.

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Cell-free protein synthesis, rapid translation system (RTS) vectors

Supplier: biotechrabbit

Expression vectors for PCR-based generation of linear templates.

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Polyadenylic acid (poly-A)

Supplier: Merck

Polyadenylic acid (poly-A) is used to evaluate binding on cationic liposomes doped with non-ionic nucleolipids. Poly-A is used in small molecule mRNA targeted drug development to evaluate the binding of potential therapeutic agents such as the isoquinoline group of alkaloids.

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pCMV-tag vectors

Supplier: STRATAGENE

The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.