Protein Purification

Supplier: Avantor

The J.T.Baker^® BAKERBOND^® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.

Supplier: Avantor

J.T.Baker® BAKERBOND® supported liquid extraction (SLE) plates are an ideal and faster alternative to traditional liquid-liquid extraction methods and allow excellent recoveries of analytes from biological matrices.

Supplier: Avantor

J.T.Baker® BAKERBOND® protein precipitation plates enable fast protein removal from biological samples.

Supplier: Merck

50% aqueous suspension in 1 M NaCl.

Supplier: Avantor

Silica-based media used for the purification of proteins based on differential salt induced hydrophobicity. Mixed mode provides unique selectivity with its hydrophobic interaction and ion exchange function.

Supplier: Abcam

Abcam's Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.

Supplier: Abcam

HRP Conjugate Stabilizer - LifeXtend (ab270548) is a proprietary multi-component reagent system that protects antibody–HRP conjugates from instability, microbrial attack, and denaturation, thus ensuring the best possible performance in experiments performed at room temperature.

Supplier: Abcam

GTP-Agarose (ab270533) comprises GTP attached to agarose beads.

Supplier: Abcam

Efficient mouse antibody purification from tissue culture supernatants.

Supplier: G-Biosciences

Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

Supplier: Abcam

Our BSA Removal Kit - Nanoparticles (ab204912) is a simple one-step, 10 minute method that effectively separates BSA from the antibody.

Supplier: Merck

Ion Exchanger I is a strong acid cation exchangers widely used in separation of noble earths and amino acids, water treatment, analysis and food industry. The bond strength of equivalent ions increases with decreasing diameter of the hydrated ion.

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128745 quickly removes these contaminants.

Supplier: Abcam

Anti-HA Affinity Resin - Amintra (ab270603) is designed for simple, rapid HA-tagged recombinant protein purification and immunoprecipitation.

Supplier: Abcam

TCS Antibody Purification Kit - Nanoparticles (ab270554) is designed for the purification of IgG fractions from tissue culture supernatants.

Supplier: Abcam

His-Tag Protein Purification Column (Pre-packed, 5 x 5ml) - AminTrap (ab270529) is a simple and easy ready to use chromatography medium for purifying His-tagged proteins that have been expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells.