1314 Results for: "qPCR"

Supplier: Quantabio

qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

Supplier: AAT BIOQUEST

A real-time polymerase chain reaction (real-time PCR), also known as quantitative polymerase chain reaction (qPCR), is a popular laboratory technique of molecular biology based on the polymerase chain reaction (PCR).

Supplier: Thermo Fisher Scientific

The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: PRIMERDESIGN

The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
Freeze dried qPCR reagents stored and shipped at ambient temperatures:

Supplier: THERMO ABGENE

ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: AAT BIOQUEST

Helixyte™ Gold is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Gold (SYBR® is the trademark of ThermoFisher).

Supplier: AAT BIOQUEST

Although ROX dyes have strong fluorescence they are notoriously unstable.

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: AAT BIOQUEST

The TAMRA dye qPCR calibration plate can be used to calibrate and maintain the 7500 real-time PCR system with fast 96-well block.

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: AAT BIOQUEST

ROX Reference Dye is equivalent to 12223-012 (Life Technology).

Supplier: THERMO ABGENE

ABsolute Blue™ Master Mix incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy and foolproof. Applications include gene expression, siRNA validation, SNP typing and genotyping.

Supplier: AAT BIOQUEST

Although ROX dyes have strong fluorescence they are notoriously unstable.

Supplier: AAT BIOQUEST

Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher).

Supplier: Corning

Axygen®, Microplate seal, Heat sealing film/foil/tape (96/384 wells), Single sheet/mat/strip, Sealing film for Axygen® PlateMax™ heat sealer, permanent, ultra clear, for qPCR, PlateMax™ ultra clear permanent heat sealing film for qPCR, Non sterile

Supplier: AAT BIOQUEST

A Cy5 dye qPCR calibration plate is a type of plate used in quantitative PCR (polymerase chain reaction) experiments.

Supplier: AAT BIOQUEST

The FAM dye qPCR calibration plate can be used to maintain the 7500 real-time PCR system with fast 96-well block.

Supplier: AAT BIOQUEST

The JUN dye qPCR calibration plate is designed for use with the 7500 real-time PCR system featuring a fast 96-well block.

Supplier: Thermo Fisher Scientific

The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by in vitro evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Supplier: MP BIOMEDICALS

Myco-Sniff-Valid™ mycoplasma PCR detection it provides a highly sensitive, specific and rapid method for the detection of a broad range of mycoplasma species in cell cultures.

Supplier: AAT BIOQUEST

The ROX dye qPCR calibration plate can be used to maintain the 7500 real-time PCR system with fast 96-well block.

Supplier: AAT BIOQUEST

The ABY dye qPCR calibration plate is designed for use with the 7500 real-time PCR system featuring a fast 96-well block.

Supplier: THERMO ABGENE

The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

Supplier: AAT BIOQUEST

The SYBR dye qPCR calibration plate can be used to maintain the 7500 real-time PCR system with fast 96-well block.

Supplier: AAT BIOQUEST

The VIC dye qPCR calibration plate can be used to maintain the 7500 real-time PCR system with fast 96-well block.

Supplier: AAT BIOQUEST

The NED dye qPCR calibration plate can be used to maintain the 7500 real-time PCR system with fast 96-well block.

Supplier: AAT BIOQUEST

6-ROX is predominately used as a reference dye for performing PCR detections.

Supplier: BRAND

For medium and high sample throughput, these 96-well plates are the efficient solution for PCR and qPCR.

Supplier: Thermo Fisher Scientific

Maxima™ Reverse Transcriptase is an advanced enzyme derived by in vitro evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.