"Quantabio"
sparQ PureMag beads
Supplier: Quantabio
sparQ PureMag beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.
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AccuStart™ II GelTrack PCR SuperMix
Supplier: Quantabio
AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
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cDNA synthesis, qScript™ Ultra SuperMix
Supplier: Quantabio
Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.
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DNA prep for PCR - tissue, Extracta™
Supplier: Quantabio
The Extracta™ DNA prep for PCR - tissue consists of a two component reagent kit for rapid and efficient extraction of PCR-ready genomic DNA from mammalian tissues. The kit has been demonstrated to work with mouse tail biopsies, ear punches and a variety of human tissue samples including hair, buccal cells and saliva.
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cDNA synthesis kits, qScript™
Supplier: Quantabio
qScript™ cDNA synthesis kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for sensitive and reliable cDNA synthesis over a broad dynamic range of input RNA. cDNA synthesis reagents are provided in a variety of SuperMix and kit formats to suit specific applications.
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qPCR cycler, Q
Supplier: Quantabio
Q qPCR cycler uses a patented magnetic induction technology to rapidly heat samples, coupled with fan-forced air for cooling to acquire data in only 25 minutes. Available as four channel models, the robust optical system acquires all channels simultaneously and allows for running the fastest multiplexed assays.
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sparQ mRNA-Seq Kit
Supplier: Quantabio
Ultra-sensitive mRNA library prep workflow with efficient poly(A) capture.
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PerfeCTa® qPCR FastMix™ reaction mixes
Supplier: Quantabio
FastMixes include buffers and stabilisers optimised for maximum PCR efficiency, reproducibility, and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. A key component of PerfeCTa® qPCR FastMixes is AccuFast™ Taq DNA polymerase that enables instant activation and rapid polymerisation kinetics. Upon heat activation (20 seconds at 95 °C), the antibodies irreversibly denature releasing fully active, unmodified Taq DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. FastMixes deliver maximum PCR efficiency, sensitivity, specificity, and a robust fluorescent signal with either fast or conventional cycling conditions.
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One-Step Fast qRT-PCR kits, qScript™
Supplier: Quantabio
These kits offer a convenient and highly sensitive solution for Real-Time reverse transcriptase PCR (qRT-PCR) of RNA templates using hybridisation probe detection chemistries, such as TaqMan® 5'-hydrolysis probes.
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PerfeCTa® qPCR FastMix® II
Supplier: Quantabio
PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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UDI adapters (1-96), sparQ
Supplier: Quantabio
sparQ UDI Adapters (1-96) are unique dual-indexed adapters designed to be used on illumina sequencing platforms. It is compatible with both DNA and RNA libraries. The use of UDI’s minimises index hopping events while also enabling greater multiplexing on flow cells.
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DNA Library Prep Kit, sparQ
Supplier: Quantabio
An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.
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One-Step SYBR® Green qRT-PCR, qScript™
Supplier: Quantabio
qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.
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AccuStart™ master mixes, long range SuperMix
Supplier: Quantabio
Superior sensitivity and multiplexing for DNA amplification of long targets.
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PerfeCTa® PreAmp 5X SuperMix
Supplier: Quantabio
PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.
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DNA fragment and library prep kit, sparQ
Supplier: Quantabio
The sparQ DNA Frag and Library Prep kit optimises the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimising DNA over fragmentation while greatly simplifying library.
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