27707 Results for: "G BIOSCIENCES"

Supplier: G BIOSCIENCES

Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Supplier: G BIOSCIENCES

E-64 is an irreversible inhibitor of cysteine proteases. It interacts with the Sn subsites of proteases but has no action on cysteine residues in other proteins.

Supplier: G BIOSCIENCES

FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. Complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions in traditional silver staining kits inhibit proteolytic digestion. In addition, glutaraldehyde, a common sensitiser in silver stains, modifies peptide lysine residues preventing complete digestion and recovery.

Supplier: G BIOSCIENCES

InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

Supplier: G BIOSCIENCES

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G BIOSCIENCES

Protein assay, Resorufin-Casein protease substrate

Supplier: G BIOSCIENCES

The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.

Supplier: G BIOSCIENCES

SDS is an anionic detergent molecule tightly bind with the protein molecule masking their native charge and rendering the protein molecules with an overall negative charge.

Supplier: G BIOSCIENCES

3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)

Supplier: G BIOSCIENCES

HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.

Supplier: G BIOSCIENCES

This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.

Supplier: G BIOSCIENCES

The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

Supplier: G BIOSCIENCES

BT Lab systems horizontal gel electrophoresis set's abundant buffer not only guarantees cooling effects, but also keeps the pH value stable during operation.

Supplier: G BIOSCIENCES

Immunotag™ rat HbA1C (glycosylated hemoglobin/hemoglobin A1c) ELISA kit, 1×96-well plate

Supplier: G BIOSCIENCES

Immunotag™ Human ROMO1 (Reactive oxygen species modulator 1) sandwich ELISA kit in 96-well plate format.

Supplier: G BIOSCIENCES

Tested against both sensitive and resistant cells.

Supplier: G BIOSCIENCES

Mode of action and anti-microbial spectrum streptomycin sulphate is an aminoglycoside antibiotic and has a bactericidal action against many gram-negative bacteria. It binds to the #12 protein on the ribosomal 30S subunit and inhibits protein synthesis.

Supplier: G BIOSCIENCES

Trypsin digestion mix provides optimal buffered conditions for in gel trypsin digestion of proteins. Suitable for 100 protein spots.

Supplier: G BIOSCIENCES

FOCUS™ Protein Reductant Kit is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.

Supplier: G BIOSCIENCES

Recom ProteaseArrest™ is a broad range, bacterial, 100X concentrated, ready to use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Recom ProteaseArrest™ inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

Supplier: G BIOSCIENCES

2-Mercaptoethanol

Supplier: G BIOSCIENCES

Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

Supplier: G BIOSCIENCES

Ampicillin sodium salt

Supplier: G BIOSCIENCES

GET™ AGAROSE DNA method involves release of DNA fragments from gel pieces followed by capture of nucleic acids on the GET™ spin columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer.

Supplier: G BIOSCIENCES

Acrylamide (electrophoresis grade) is supplied as a powder or a 40% solution in ultrapure water. Bisacrylamide (Bis (N,N’-methylenebisacrylamide)) (electrophoresis grade) is supplied as a powder or a 2% solution in ultrapure water.

Supplier: G BIOSCIENCES

di-Potassium hydrogen phosphate 1 mol/l in aqueous solution

Supplier: G BIOSCIENCES

G-Biosciences' Coomassie Brilliant Blue (CBB) G-250 and R-250 Stain are based on a colloidal Coomassie stain. CBB G-250 and R-250 stain proteins with high band visibility. The staining of gels with CBB G-250 and R-250 allows the examination of protein bands even during the staining process.

Supplier: G BIOSCIENCES

G-Biosciences' Coomassie Brilliant Blue (CBB) G-250 and R-250 Stain are based on a colloidal Coomassie stain. CBB G-250 and R-250 stain proteins with high band visibility. The staining of gels with CBB G-250 and R-250 allows the examination of protein bands even during the staining process.

Supplier: G BIOSCIENCES

TRITON™ X-100 (Polyethylene glycol tert-octylphenyl ether)

Supplier: G BIOSCIENCES

FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.