27707 Results for: "G BIOSCIENCES"

Supplier: G BIOSCIENCES

InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.

Supplier: G BIOSCIENCES

Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.

Supplier: G BIOSCIENCES

SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

Supplier: G BIOSCIENCES

Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.

Supplier: G BIOSCIENCES

Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.

Supplier: G BIOSCIENCES

Sulfo SASD Sulfosuccinimidyl-2-(p-azidosalicylamido)ethyl-1,3-dithiopropionate

Supplier: G BIOSCIENCES

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G BIOSCIENCES

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G BIOSCIENCES

The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.

Supplier: G BIOSCIENCES

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.

Supplier: G BIOSCIENCES

G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.

Supplier: G BIOSCIENCES

Anti-IgG Goat Antibody (HRP (Horseradish Peroxidase))

Supplier: G BIOSCIENCES

EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.

Supplier: G BIOSCIENCES

N-Sulfosuccinimidyl(4-iodoacetyl)aminobenzoate Heterobifunctional Cross Linkers.

Supplier: G BIOSCIENCES

3,3’-Dithiobis(sulfosuccinimidyl propionate) Homobifunctional Cross Linker.

Supplier: G BIOSCIENCES

Anti-IgG Rabbit Antibody (HRP (Horseradish Peroxidase))

Supplier: G BIOSCIENCES

m-Maleimidobenzoyl-N-hydroxysulfosuccinimide esterHeterobifunctional Cross Linker

Supplier: G BIOSCIENCES

N-(ρ-Maleimidophenyl) isocyanate Heterobifunctional Cross Linkers.

Supplier: G BIOSCIENCES

Disuccinimidyl suberate Homobifunctional Cross Linker.

Supplier: G BIOSCIENCES

Calcium chloride 1 M in aqueous solution

Supplier: G BIOSCIENCES

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

Supplier: G BIOSCIENCES

TE Buffer (Tris-EDTA Buffer) is a common molecular biology buffer that is used to protect DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.

Supplier: G BIOSCIENCES

Potassium hydroxide 2 mol/l (2 N) in aqueous solution

Supplier: G BIOSCIENCES

N-Octyl-β-D-glucopyranoside

Supplier: G BIOSCIENCES

Tween® 20 (Polysorbate) 10% in aqueous solution, Proteomics Grade

Supplier: G BIOSCIENCES

G-Biosciences’ Proteomic Grade Detergent Solutions contain reduced peroxides and carbonyl compounds. In addition, the detergents have less than 50µS conductivity. These detergents are offered as sterile, 10% aqueous solutions, sealed under inert gas, and are suitable for protein applications. These non-ionic detergents are suitable for isolating membrane-protein complexes.

Supplier: G BIOSCIENCES

Brij® 35 10% in aqueous solution, Proteomics Grade

Supplier: G BIOSCIENCES

The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: G BIOSCIENCES

The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

Supplier: G BIOSCIENCES

A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.