27707 Results for: "G BIOSCIENCES"
Phosphate buffered saline (PBS), 10X
Supplier: G BIOSCIENCES
PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.
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Water, sterile, distilled
Supplier: G BIOSCIENCES
Laboratory reagent grade, sterile distilled water for your laboratory needs. This water is purified by steam distillation and sterile filtered for use as a research laboratory reagent. The distilled water is low endotoxin <0.025EU/ml and low conductivity (<10uS). This water is ideal for the preparation of reagents, rinsing of glassware and plasticware, and various other laboratory and research applications. No toxic agents such as DEPC are included in the preparation of distilled water, eliminating interference with enzymatic analysis.
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Sodium azide 1% in aqueous solution
Supplier: G BIOSCIENCES
Sodium azide 1% in aqueous solution
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Empty FPLC columns, G-TRAP™
Supplier: G BIOSCIENCES
These empty columns are designed for fast protein liquid chromatography.
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Buffer, PBS solution 10X concentrate (phosphate buffered saline)
Supplier: G BIOSCIENCES
10X PBS (Phosphate Buffered Saline) is a 10 fold concentrated, sterile, pH adjusted blend of phosphate buffer and saline solution, which when diluted 1 to 10 in ultrapure water produces a phosphate buffered saline of 8mM NaH2PO4, 150mM NaCl, 3mM KCl, and 2mM KH2PO4 at pH 7.4.
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Accessories for protein kits
Supplier: G BIOSCIENCES
LongLife™ Zymolyase®, 1,5 U/µl
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Water, Molecular Grade DNase-, RNase-free
Supplier: G BIOSCIENCES
Molecular Grade Water is ultrapure water that certified RNase, DNase and Protease free, is suitable for use in molecular biology applications, which demand a high quality of water and assurance that the water is free from nucleases and protease contamination. No toxic agents, such as DEPC, are used in the manufacturing of Molecular Grade Water, eliminating DEPC interference of enzymatic reactions.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline)
Supplier: G BIOSCIENCES
The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.
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Sodium chloride 0.9% in water normal saline solution
Supplier: G BIOSCIENCES
G-Biosciences offers 0.9% NaCl in two convenient sizes to meet your particular laboratory needs. These sterile saline solutions are suitable for numerous applications.
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Experimentation kits, bacterial gram staining
Supplier: G BIOSCIENCES
The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.
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Protease inhibitor cocktail, Bacterial ProteaseArrest™
Supplier: G BIOSCIENCES
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Bacterial ProteaseArrest™ inhibits bacterial serine, cysteine and other bacterial specific proteases, including aminopeptidases and aspartic proteases.
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Micro Dialysis System, Tube-O-Dialyzer™
Supplier: G BIOSCIENCES
'NO-LOSS' dialyser.
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Lysates, mammalian GenLysate™, human
Supplier: G BIOSCIENCES
GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
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Sample clean up, Perfect-FOCUS™
Supplier: G BIOSCIENCES
Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.
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Lysates, mammalian GenLysate™, rat
Supplier: G BIOSCIENCES
GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
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Accessories for protein assays
Supplier: G BIOSCIENCES
Protein assay, Bovine γ-Globulin Protein Standard (2 mg/ml)
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Protein concentration kit, Column-PROTEIN-Concentrate™
Supplier: G BIOSCIENCES
The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.
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tri-Sodium citrate dihydrate ACS
Supplier: G BIOSCIENCES
tri-Sodium citrate dihydrate ACS
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Phosphatase assay kit
Supplier: G BIOSCIENCES
The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.
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2-Iminothiolane hydrochloride (Traut's Reagent)
Supplier: G BIOSCIENCES
Traut's Reagent is used for the preparation of disulfide bridges or for the generation of sulphhydryl groups for conjugation. This is a small, water-soluble compound that reacts with primary amines under mild conditions (pH 7 to 10, 25 °C) for thiolation (sulphhydryl addition).
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GET™ CLEAN DNA Kit
Supplier: G BIOSCIENCES
GET™ CLEAN DNA uses high binding affinity GET™ spin columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100 bp to 20 kb. GET™ spin columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.
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Genomic DNA isolation from bacteria, OmniPrep™ for Gram Positive Bacteria kit
Supplier: G BIOSCIENCES
OmniPrep™ for Gram Positive Bacteria Kit isolates high quality genomic DNA from gram-positive bacteria. If used according to the protocols, this kit purifies DNA from 100 ml gram-positive bacteria culture.
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Protease Screening Kit
Supplier: G BIOSCIENCES
The Protease Screening Kit provides a simple and quick method for testing samples for proteolysis. Simply incubate sample in the reagent provided and obtain results.
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Protein fractionation kit, FOCUS™ mitochondria
Supplier: G BIOSCIENCES
FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
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Albumin removal kit, AlbuminOUT™
Supplier: G BIOSCIENCES
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.
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Experimentation kits, physical properties of proteins
Supplier: G BIOSCIENCES
The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.
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Proteome kits, FOCUS™ Bacterial Proteome
Supplier: G BIOSCIENCES
Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from E.coli, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
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Experimentation kits, mutation detection and analysis
Supplier: G BIOSCIENCES
A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
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Experimentation kits, nucleic acid quantification
Supplier: G BIOSCIENCES
The kit utilises the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.
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Human cancer cell line blot
Supplier: G BIOSCIENCES
Ready to screen human cancer cell line blots.