237756 Results for: "Cloning+Kits+and+Ligation"

Supplier: NOVAGENE

Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.

Supplier: Thermo Fisher Scientific

The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Supplier: Thermo Fisher Scientific

The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in E. coli. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to E. coli cells.

Supplier: SYNTHETIC GENOMICS

Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Supplier: SYNTHETIC GENOMICS

The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

Supplier: SYNTHETIC GENOMICS

The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

Supplier: Thermo Fisher Scientific

This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

Supplier: Cytiva

T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.

Supplier: SYNTHETIC GENOMICS

The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

Supplier: SYNTHETIC GENOMICS

The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

Supplier: Oxford Nanopore Technologies

Ligation Sequencing Kits enable ligation-based, PCR-free nanopore sequencing of native DNA.

Supplier: SYNTHETIC GENOMICS

Gibson Assembly® Positive Control, 5 reactions

Supplier: VWR Chemicals

Cloning Buffer, SM BUFFER

Supplier: VWR Chemicals

Cloning Buffer, EDTA, 0,5 M solution

Supplier: VWR Chemicals

Cloning Buffer, TRIS buffer solution pH 9 (1 mol/l)

Supplier: VWR Chemicals

With diethyl pyrocarbonate (DEPC).

Supplier: CAYMAN

Cloning Buffer, TRIS buffer solution pH 7 (1 mol/L)

Supplier: VWR Chemicals

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l) for biotechnology

Supplier: PanReac AppliChem

Cloning Buffer, TRIS buffer solution (1 mol/l)

Supplier: VWR Chemicals

Ready to use Tris-EDTA buffer.

Supplier: Thermo Fisher Scientific

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: CAYMAN

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/L)

Supplier: G BIOSCIENCES

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: CAYMAN

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: CAYMAN

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

Supplier: NOVUS BIOLOGICALS

10X Tris-EDTA buffer pH 9.0.

Supplier: Thermo Fisher Scientific

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: PanReac AppliChem

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: CAYMAN

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: ROCKLAND

This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.