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Human Tagged ORF Clone, CFTR (NM_000492)
Human Tagged ORF Clone, CFTR (NM_000492)
Catalog # ORIGRG216476
New Product
CAS Number:  
Human Tagged ORF Clone, CFTR (NM_000492)
Catalog # ORIGRG216476
New Product
CAS Number:  

Specifications

  • Description:
    CFTR (NM_000492) human tagged ORF clone, E. coli selection: Ampicillin (100 µg/ml) mammalian cell selection: Neomycin
  • Storage temperature:
    −20 °C
  • Vector details:
    pCMV6-AC-GFP (PS100010)

Specifications

About this item

This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

  • Centrifuge at 5000×g for 5 mins

  • Carefully open the tube and add 100 µl of sterile water to dissolve the DNA
  • Close the tube and incubate for 10 minutes at room temperature
  • Briefly vortex the tube and then do a quick spin (less than 5000×g) to concentrate the liquid at the bottom
  • Store the suspended plasmid at −20 °C. The DNA is stable for at least one year from date of shipping when stored at −20 °C

The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10 µg of transfection-ready, dried plasmid DNA (reconstitute with 100 µl of water).