Human Tagged ORF Clone, CFTR (NM_000492)
Catalog # ORIGRG216476
Supplier: ORIGENE TECHNOLOGIES
New Product
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Specifications
- Description:CFTR (NM_000492) human tagged ORF clone, E. coli selection: Ampicillin (100 µg/ml) mammalian cell selection: Neomycin
- Storage temperature:−20 °C
- Vector details:pCMV6-AC-GFP (PS100010)
Specifications
About this item
This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Centrifuge at 5000×g for 5 mins
- Carefully open the tube and add 100 µl of sterile water to dissolve the DNA
- Close the tube and incubate for 10 minutes at room temperature
- Briefly vortex the tube and then do a quick spin (less than 5000×g) to concentrate the liquid at the bottom
- Store the suspended plasmid at −20 °C. The DNA is stable for at least one year from date of shipping when stored at −20 °C
The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10 µg of transfection-ready, dried plasmid DNA (reconstitute with 100 µl of water).