- Pk:0,05 mg
- Protein/peptide type:Recombinant
- Source:Cells
- Species:Human
- Tag sequence:C-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimise freeze-thaw cycles.
- Endotoxin content:<0,1 ng/µg (1 IEU/µg) as determined by LAL test
- Protein synonyms:25 kDa alpha-2-microglobulin-related subunit of MMP-9|Neutrophil gelatinase-associated lipocalin|Lipocalin-2|HNL|LCN2|Siderocalin LCN2|NGAL|Oncogene 24p3|p25
- Protein/peptide name:NGAL
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:21,6 kDa
- Sequence:Gln21-Gly198
- Formulation:Supplied as a 0,2 µm filtered solution of PBS, 50% glycerol, pH 7,4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the Lyophilised protein in ddH₂O. Please aliquot the reconstituted solution to minimise freeze-thaw cycles.
- Tested applications:Used for biological assays
LCN2 is iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. LCN2 binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. LCN2 is involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. LCN2 is involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
- Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays.