A simple, reproducible, and sensitive tool for assaying free thiol content in samples.

• Measure free thiol content in plasma, serum, urine, cell lysates, and tissue homogenates
• Assay 40 samples in duplicate
• Plate-based fluorometric measurement (ex 380 to 390 nm, em 510 to 520 nm)
• Includes both a glutathione and cysteine standard
• Range: 15 nm to 1 µm

The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. Cayman’s Thiol detection provides a simple, reproducible, and sensitive tool for assaying free thiol content in samples (i.e., plasma, serum, tissue homogenates, cell lysates, and urine). The assay utilises a proprietary fluorometric detector that reacts with thiol groups to emit a strong fluorescent signal which can be detected using excitation wavelengths between 380 to 390 nm and emission wavelengths between 510 to 520 nm.