Rebaudioside D, a glycoside of the diterpene steviol is a minor constituent found in leaves of Stevia rebaudiana. It is considered non-caloric and is about 200 to 220 times sweeter than sucrose.

It may be used as internal standard to optimise the desorption electrospray ionisation (DESI) mass spectrometry during the analysis of Stevia leaves for diterpene glycosides.