Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

• Reaction set up can be done at room temperature
• Reduces non-specific amplification and prevents primer degradation during set up
• Zero-time reactivation due to hot start technology
• Speed of the polymerase allows short extension times
• Robust reactions, minimal optimisation needed
• Increased product yields with minimal enzyme amounts
• Direct loading on gel with Phusion® green buffer