NPP ELISA alkaline phosphatase substrate

NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.

Phosphatase-conjugated detection antibodies can act upon the pNPP to convert it to a yellow para-nitrophenol. This yellow product can be measured on a spectrophotometer at 405 nm.

: Diluent buffer NPP-B500 for NPP-10 has to be ordered separately.

: Both the substrate and buffer have to be stored at 4 °C prior to opening and should be protected from moisture and light.

NPP-B500_

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Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC

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Water, HiPerSolv CHROMANORM® for HPLC

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VWR® Facial Tissues

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Acetonitrile ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

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VWR®, Nitrile Gloves

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Acetone ≥99%, TECHNICAL

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Sealing film for manual application, Parafilm® M

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Acetonitrile ≥99.9%, LiChrosolv® Reag. Ph. Eur., gradient grade for liquid chromatography, Supelco®

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Acetonitrile, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments

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VWR®, Transfer Pipettes, High Performance

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VWR® NITRILE LIGHT, Nitrile Gloves

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VWR® Serological Pipette, Standard Line

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Nitrile gloves, Kimtech™ Purple Nitrile™

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