You searched for: Western Blotting Reagents
Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Biotin random prime DNA labelling kit, North2South™
Supplier: Thermo Fisher Scientific
North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.
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NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™
Supplier: Thermo Fisher Scientific
QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.
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CN/DAB substrate kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.
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Pierce™ Western Blot Signal Enhancer
Supplier: Thermo Fisher Scientific
Western blot signal enhancer is a membrane treatment that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a Level at which it could not be detected without enhancement.
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Far-western blot kit for biotinylated proteins, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins through interaction with any biotin-labeled test protein used as the primary probe. On-membrane detection offers greater sensitivity whereas in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer.
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Western Blotting Substrate, Pierce™ ECL Plus
Supplier: Thermo Fisher Scientific
The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
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SuperSignal™ Pierce™ West Pico PLUS chemiluminescent substrate
Supplier: Thermo Fisher Scientific
SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by Western blot analysis.
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Western blotting kits, SuperSignal™ west pico chemiluminescent substrate kits, Pierce™
Supplier: Thermo Fisher Scientific
These kits include SuperSignal™ West Pico Substrate that is a highly sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. The kits also contain reagents for the specific detection of biotinylated antibodies, rabbit IgG or mouse IgG. The substrate’s intense signal output enables detection of picogram amounts of antigen. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.
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Pierce™ Biotin 3' End DNA Labelling Kit
Supplier: Thermo Fisher Scientific
This Biotin 3' End DNA Labelling Kit is used for tagging single-stranded DNA primers with biotin for use in non radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.
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Fluorogenic peroxidase substrate kit, QuantaBlu™
Supplier: Thermo Fisher Scientific
QuantaBlu™ Fluorogenic peroxidase substrate kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.
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SuperSignal™ Protein ImmunoDetection Reagents, ELISA Femto Maximum Sensitivity Substrate
Supplier: Thermo Fisher Scientific
SuperSignal® ELISA Femto maximum sensitivity substrate utilises the SuperSignal® system with an enhancer formulation for superior protein detection and low-end linearity in ELISA applications. Ideal for automated high-throughput screening applications.
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Restore™ Western Blot Stripping Buffer
Supplier: Thermo Fisher Scientific
Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.
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Chemiluminescent RNA EMSA kit, LightShift™
Supplier: Thermo Fisher Scientific
LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
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Pierce™ Firefly Signal Enhancer (100X)
Supplier: Thermo Fisher Scientific
Pierce™ Firefly Signal Enhancer (100X) is designed to complement the Firefly Luciferase Flash and Glow assay and is recommended when maximum sensitivity is desired.
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Pierce™ OPD (o-phenylenediamine Dihydrochloride)
Supplier: Thermo Fisher Scientific
OPD (o-phenylenediamine dihydrochloride) is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492 nm by ELISA plate readers.
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Western blot kits, TrueBlot®
Supplier: ROCKLAND
TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.
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DAB substrate kit, metal enhanced, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.
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Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit
Supplier: Thermo Fisher Scientific
The Pierce™ Chemiluminescent nucleic acid detection module is a complete system for the detection of biotin-labeled nucleic acids for various blotting applications, including Northern/Southern blots, ribonuclease protection assays (RPAs), and electrophoretic mobility shift assays (EMSAs) or gel-shift assays.
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Western blotting blocking reagent, Amersham™ ECL™
Supplier: Cytiva
Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents
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CheLuminate-HRP PicoDetect Chemiluminescent Detection Kit
Supplier: PanReac AppliChem
The CheLuminate-HRP PicoDetect is a complete kit with ready to use reagents for chemiluminescent detection of immobilised proteins (Western blot) or immobilised nucleic acids (Southern or Northern blot), conjugated with horseradish peroxidase (HRP) directly or indirectly.
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Amersham™ ECL™ Western Blotting Detection Reagent, Cytivia
Supplier: Cytiva
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
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Sample clean up, PAGE-Perfect™
Supplier: G BIOSCIENCES
Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.
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Saponin permeating solution, 0,5% w/v solution in PBS (10X)
Supplier: Thermo Fisher Scientific
Saponin permeating solution, is used as a cell permeabilising agent.
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Sample clean up, Perfect-FOCUS™
Supplier: G BIOSCIENCES
Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.
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SSC (20X), RNAse free
Supplier: Thermo Fisher Scientific
SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.
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Chemiluminescent super sensitive HRP substrate, FemtoMax™
Supplier: ROCKLAND
FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.
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Western blotting blocking reagent, Amersham ECL™ Prime
Supplier: Cytiva
Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
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Luminol and oxidising solutions
Supplier: Thermo Fisher Scientific
For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.
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Western Blotting Detection Reagent
Supplier: MILLIPORE
Western Blotting Detection Reagent
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Chemiluminescent western blot kits, FemtoMax™
Supplier: ROCKLAND
These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.