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Electrophoresis Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

Phosphate-buffered saline (PBS, 10X), pH 7,4, for Western blot

Supplier: Thermo Fisher Scientific

Washing buffer, Phosphate-buffered saline (PBS, 10X)

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TRIS-buffered saline (TBS, 10X) pH 7,6

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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TBE sample buffer (6X)

Supplier: Thermo Fisher Scientific

Loading Buffer, TBE Sample Buffer, 6X

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TRIS-glycine-SDS running buffer (10X), pH 8,3

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-SDS running buffer (10X)

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TRIS-glycine native sample buffer (4X), pH 8,6

Supplier: Thermo Fisher Scientific

Loading Buffer, TRIS-Glycine Native Sample Buffer (4X)

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Buffer, MOPS solution 10X concentrate

Supplier: LONZA

Running Buffer, MOPS buffer solution 10X concentrate

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TRIS-buffered saline (TBS, 10X) pH 7,4, for Western blot

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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Tween 20 washing buffer, 0,5% in PBS (10X)

Supplier: Thermo Fisher Scientific

Washing buffer, Tween 20 washing buffer, 0,5% in PBS (10X)

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Tween 20 washing buffer, 2% in PBS (20X)

Supplier: Thermo Fisher Scientific

Washing buffer, Tween 20 washing buffer, 2% in PBS (20X)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: CALBIOCHEM

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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TRIS-glycine-native running buffer (10x), pH 8,5

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-native running buffer (10x)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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TAE buffer, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.

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Blocking reagents, Blotto

Blocking reagents, Blotto

Supplier: ROCKLAND

Contains immunoanalytical grade non-fat dry milk. Blocks non specific binding of proteins in various immunological assays. Ideal for use in western blotting and ELISA methods.

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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Buffers, TBS, 20X solution, pH 7,4, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Used in Western blotting protocols.

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TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer), MP Biomedicals

Supplier: MP BIOMEDICALS

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

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DNA gel loading buffer 6X

DNA gel loading buffer 6X

Supplier: LONZA

This buffer is a Ficoll based loading buffer containing Ficoll®, EDTA, xylene cyanol, and bromophenol blue. Ideal for separation of DNA fragments with improved resolution.

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Alkaline running buffer solution

Supplier: Thermo Fisher Scientific

Running Buffer, Alkaline running buffer solution

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MES-SDS running buffer (20X)

Supplier: Thermo Fisher Scientific

Running Buffer, MES-SDS running buffer (20X)

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MOPS-SDS running buffer (20X)

Supplier: Thermo Fisher Scientific

Running Buffer, MOPS-SDS running buffer (20X)

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TRIS-buffered saline (TBS, 10X) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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TRIS-buffered saline (TBS, 20X) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X)

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TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Supplier: Corning

20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures

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