You searched for: Electrophoresis Buffers
The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5
Supplier: ROCKLAND
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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Blocking buffer, fish gelatin
Supplier: BIOTIUM
Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: BIOTIUM
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: G BIOSCIENCES
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: ROCKLAND
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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SDS-Page running gel buffers, 10X concentrate
Supplier: ROCKLAND
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.
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Immunoassay Blocking (BSA Free)
Supplier: ABCAM
Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.
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In-situ hybridisation buffer for HPV probes
Supplier: ENZO LIFE SCIENCES
In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.
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Protein blocking buffers, Superior™
Supplier: G BIOSCIENCES
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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Buffer, PBS (10X, Dulbecco's) powder pack
Supplier: PanReac AppliChem
Powder mixture to prepare PBS buffer (10X) according to Dulbecco.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline)
Supplier: G BIOSCIENCES
The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.
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WB blocking buffer kit, TrueBlack®
Supplier: BIOTIUM
The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).
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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4
Supplier: CAYMAN
250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.
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Protein blocking agent, BLOT-QuickBlocker™
Supplier: G BIOSCIENCES
BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.
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SSPE Buffer, 20X concentrate, pH 7,4
Supplier: ROCKLAND
20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.
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TAE buffer, 50X
Supplier: EDVOTEK
This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.
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TAE buffer (20X), Pierce™
Supplier: Thermo Fisher Scientific
20X TAE buffer is a space-saving stock solution that is ideal for casting and preparing Tris-Acetate EDTA running buffer used for agarose gel electrophoresis of nucleic acids.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: OMEGA BIOTEK
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Wash buffer, Pierce™
Supplier: Thermo Fisher Scientific
These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.
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Pierce™ BupH™ Wash Buffers, Dry Blend Pack
Supplier: Thermo Fisher Scientific
BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.
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Blocking solution, BLOK™ Casein
Supplier: G BIOSCIENCES
BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.
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Pierce™ Lane Marker Non-reducing Sample Buffer (5X)
Supplier: Thermo Fisher Scientific
Pierce™ Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.
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Blocker™ Blocking Buffer, BLOTTO in TBS
Supplier: Thermo Fisher Scientific
Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other detection methods.
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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution
Supplier: PanReac AppliChem
Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.
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Gel loading solution, 10X
Supplier: EDVOTEK
1 ml concentrate for 200 samples.
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VHB DNA Washing Buffer
Supplier: OMEGA BIOTEK
VHB buffer must be diluted with ethanol before use.
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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer) pH 8,3
Supplier: PanReac AppliChem
Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer) pH 8,3
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Wash buffer, Pierce™ Fast Wash Buffer
Supplier: Thermo Fisher Scientific
Pierce™ Fast Wash buffer, 10X, for use with Pierce™ Fast Blocking buffer.
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Tris-Glycine native gel running buffer
Supplier: G BIOSCIENCES
Running Buffer, Tris-Glycine [10X]
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: PanReac AppliChem
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)