You searched for: Protein Purification Resins
Blue Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
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Affinity chromatography media, MabSelect SuRe™ LX
Supplier: Cytiva
MabSelect SuRe™ LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes.
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CM Sephadex™ C-50 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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QAE Sephadex™ Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow
Supplier: Cytiva
Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
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Ion exchange chromatography media, ANX Sepharose™ 4 Fast Flow (High Sub)
Supplier: Cytiva
ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.
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Ion exchange chromatography media, Q Sepharose™ High Performance
Supplier: Cytiva
Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.
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Affinity chromatography media, MabSelect™ PrismA
Supplier: Cytiva
MabSelect™ PrismA has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing future demands in mAb processing to be met.
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Reversed phase chromatography media, SOURCE™ 15RPC
Supplier: Cytiva
SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.
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Gel filtration media, Sephadex™ G-100
Supplier: Cytiva
Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.
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Ion exchange chromatography media, SOURCE™ 15S
Supplier: Cytiva
SOURCE™ 15S is a synthetic high performance, preparative, chromatography medium, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.
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Gel filtration media, Sephadex™ G-75
Supplier: Cytiva
Sephadex™ G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.
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DEAE Sephadex™ A-25 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
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Affinity chromatography media, Capto™ L
Supplier: Cytiva
Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
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Superose™ 12 Gel Filtration Media, Prep Grade
Supplier: Cytiva
Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability
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SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
SP Sephadex™ C-25 is a strong cation exchanger. The ion exchange group is a sulphopropyl group which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.
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Ion exchange chromatography media, CM Sepharose™ Fast Flow
Supplier: Cytiva
CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
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Affinity chromatography media, MabSelect SuRe™
Supplier: Cytiva
MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.
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Ion exchange chromatography media, DEAE Sephacel™
Supplier: Cytiva
DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.
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2D Clean-Up kit
Supplier: Cytiva
The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.
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Ion exchange chromatography media, DEAE Sepharose™ Fast Flow
Supplier: Cytiva
DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.
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Immobilised metal ion affinity chromatography (IMAC) media, Ni Sepharose™ excel
Supplier: Cytiva
Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.
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Affinity coupling media, Epoxy-Activated Sepharose™ 6B
Supplier: Cytiva
Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.
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Chelex® 100, sodium form
Supplier: Merck
Chelex® 100 Chelating Resin is a weak cation exchange resin that has high selectivity, approximately 5,000:1, for copper, iron, and other heavy metal ions over monovalent cations. Chelex 100 Resin is made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.
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Ion exchange chromatography media, SP Sepharose® fast flow
Supplier: Merck
Sepharose Fast Flow is composed of macroporous, beaded, cross-linked agarose and modified with sulphopropyl (SP) strong cation exchange groups which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.
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Gel filtration media, Sephadex® G-50
Supplier: Merck
Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatrography and gel filtration chromatography.
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Affinity chromatography media, gelatin-agarose
Supplier: Merck
Gelatin-agarose is an agarose in saline suspension used for affinity chromatography, protein chromatography and specialty resins.
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Lectin from Triticum vulgaris, immobilized on Agarose CL-4B, for affinity chromatography, Supelco®
Supplier: Merck
50% aqueous suspension in 1 M NaCl.
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Ion exchange chromatography media, Ion Exchanger I
Supplier: Merck
Ion Exchanger I is a strong acid cation exchangers widely used in separation of noble earths and amino acids, water treatment, analysis and food industry. The bond strength of equivalent ions increases with decreasing diameter of the hydrated ion.