You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Fluorimetric Lead (II) Ion Quantitation Kit
Supplier: AAT BIOQUEST
Lead is a highly poisonous metal to human beings, especially young children.
Expand 1 Items
ReadiUse™ NADPH Regenerating Kit
Supplier: AAT BIOQUEST
NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species).
Expand 1 Items
Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit
Supplier: AAT BIOQUEST
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.
Expand 1 Items
ReadiUse™ NADP Regenerating Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. AAT Bioquest's ReadiUse™ NADP regenerating kit provides a flexible prepackaged NADP regenerating system to accommodate various experimental designs.
Expand 1 Items
Pierce™ Firefly Luc One-Step Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.
Expand 2 Items
Amplite® Fluorimetric DPP4 Inhibitor Screening Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors.
Expand 1 Items
Pierce™ Alkaline Phosphatase, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated alkaline phosphatase is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.
Expand 1 Items
Amplite® Rapid Colorimetric Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules.
Expand 1 Items
Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*
Supplier: AAT BIOQUEST
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
Expand 1 Items
Amplite® Colorimetric Biotin Quantitation Kit
Supplier: AAT BIOQUEST
The avidin/streptavidin-biotin interaction is the strongest known non-covalent biological interaction (Kd = 10 to 15 M⁻¹) between a protein and its ligand.
Expand 1 Items
Amplite® Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Acetylcholine and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways.
Expand 1 Items
Amplite® Colorimetric Malondialdehyde (MDA) Quantitation Kit
Supplier: AAT BIOQUEST
Malondialdehyde (MDA) is natural byproduct of lipid peroxidation and is widely used as a key indicator to determine the oxidative stress and free radical formation.
Expand 1 Items
Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*
Supplier: AAT BIOQUEST
Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
Expand 1 Items
β-Galactosidase Assay Kit and Reagents
Supplier: Thermo Fisher Scientific
The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
Expand 3 Items
Beadlite™ Rapid Colorimetric Thiol Quantitation Kit for Nanoparticles
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. Beadlite™ Rapid Colorimetric Thiol Quantitation kit provides a robust method to quantify thiol-functionalised nanoparticle with our proprietary Thiolite™ 490 that has its maximum absorbance at ~490 nm.
Expand 1 Items
Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Fluorescamine is intrinsically non-fluorescent but reacts rapidly with primary aliphatic amines, including those in peptides and proteins, to yield a blue-green-fluorescent derivative.
Expand 1 Items
Amplite® Rapid Colorimetric APC-Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Rapid Colorimetric APC-Maleimide Quantitation Kit facilitates the quantification of maleimide groups on maleimide-activated APC proteins using a proprietary sensor, Maleimide 555™, which has an absorption peak at ~555 nm.
Expand 1 Items
Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Screen Quest™ fluorimetric neutrophil elastase inhibitor screening kit offers a fast, sensitive, and high-throughput method for screening and characterising potential inhibitors of NE.
Expand 1 Items
Amplite® Blue Peroxidase Substrate
Supplier: AAT BIOQUEST
A variety of substrates are available for detecting peroxidase activity in ELISA assays. colourimetric HRP substrates (e.g., TMB, OPD and ABTS) have been widely used for years. Each of these substrates varies greatly with respect to its performance characteristics such as detection sensitivity, working range and attainable signal-to-noise ratios. Amplite® Blue peroxidase substrate is a soluble fluorogenic substrate for detection of peroxidase activity. Amplite® Blue allows rapid HRP detection assays to be performed with greater sensitivity than the colourimetric HRP substrates (such as TMB and OPD). The fluorescent product of Amplite® Blue does not photobleach. Amplite® Blue exhibits a flat baseline in assays, which facilitates low-level detection sensitivity and allows for high signal-to-noise ratios.
Expand 1 Items
SAM Methyltransferase assay, SAM510™
Supplier: G BIOSCIENCES
SAM510™ assay is a continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
Expand 1 Items
Protease Screening Kit
Supplier: G BIOSCIENCES
The Protease Screening Kit provides a simple and quick method for testing samples for proteolysis. Simply incubate sample in the reagent provided and obtain results.
Expand 1 Items
Fluorescent β-Galactosidase assay kit
Supplier: G BIOSCIENCES
β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.
Expand 1 Items
Active GTPase pull-down and detection kits
Supplier: Thermo Fisher Scientific
The Active GTPase Pull-Down and Detection Kits allows the monitoring of changes in GTPase activity levels by enriching and detecting the active (GTP-bound) state of a particular GTPase.
Expand 6 Items
Fluorescent β-Galactosidase assay kit
Supplier: G BIOSCIENCES
The Fluorescent β-Galactosidase assay is used for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The Fluorescent β-Galactosidase assay (MUG) provides a 96-well assay format for galactosidase activity that is suitable for high throughput applications. The production of the fluorphore is monitored at an emission/excitation wavelength of 365/460 nm.
Expand 1 Items
Kinase enrichment kits, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Kinase Enrichment Kits utilise an ActivX™ ATP or ADP probe to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.
Expand 2 Items
Superoxide dismutase assay kit
Supplier: ABNOVA
Superoxide Dismutase (SOD) Assay Kit is used for the determination of SOD in blood, cells, tissues and other biological samples.
Expand 1 Items
Alkaline phosphatase assay kit
Supplier: ABNOVA
Alkaline phosphatase assay kit allows the colorimetric kinetic determination of serum alkaline phosphatase activity.
Expand 1 Items
Second messenger quantitation assays, Biotrak™ cAMP and cGMP EIA systems
Supplier: Cytiva
Eliminate lengthy sample extraction procedures using direct assay technology.
Expand 1 Items
LOX assay kit (fluorometric)
Supplier: ABNOVA
LOX assay kit (fluorometric) is designed for the measurement of LOX.
Expand 1 Items
Catalase Assay Kit
Supplier: Merck
Catalase is an ubiquitous antioxidant enzyme that catalyses the decomposition of hydrogen peroxide (H₂O₂) to water and oxygen. By preventing excessive H₂O₂ build up, catalase allows important cellular processes which produce H₂O₂ as a byproduct to occur safely. Deficiency in catalase activity has been associated with grey hair and peroxisomal disorder acatalasia.