You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Supplier: AAT BIOQUEST
Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.
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Quantitative peroxide assay kit (lipid), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
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Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit
Supplier: AAT BIOQUEST
Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.
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Amplite® Fluorimetric Xanthine Assay Kit
Supplier: AAT BIOQUEST
Xanthine is a purine base found in most human body tissues and fluids.
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Amplite® Luminometric Peroxidase (HRP) Assay Kit
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*
Supplier: AAT BIOQUEST
Caspases play important roles in apoptosis and cell signaling.
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Amplite® Colorimetric L-Lactate Assay Kit
Supplier: AAT BIOQUEST
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric cADP-Ribose Assay Kit
Supplier: AAT BIOQUEST
This cADP-ribose assay can be performed in a convenient 96-well or 384-well microtiter plate.
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Serine hydrolase probes, ActivX™ FP
Supplier: Thermo Fisher Scientific
The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.
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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Glutamic acid is one of the 20 proteinogenic amino acids.
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Renilla luciferase glow assay kits, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce renilla Luciferase glow assay kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of green renilla luciferase reporter.
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Amplite® Colorimetric Xanthine Assay Kit
Supplier: AAT BIOQUEST
Xanthine is a purine base found in most human body tissues and fluids.
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Amplite® Fluorimetric D-Lactate Assay Kit
Supplier: AAT BIOQUEST
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit
Supplier: AAT BIOQUEST
Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.
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Renilla-firefly luciferase dual assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Renilla-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.
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Amplite® Fluorimetric Lipase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity.
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Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
Supplier: AAT BIOQUEST
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
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Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
Supplier: AAT BIOQUEST
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
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Amplite® Colorimetric NADPH Assay Kit
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit
Supplier: AAT BIOQUEST
Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway.
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Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit
Supplier: AAT BIOQUEST
Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.
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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric Antioxidant Activity Assay Kit
Supplier: AAT BIOQUEST
Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.
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Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
Supplier: AAT BIOQUEST
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
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ReadiUse™ NADP Regenerating Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. AAT Bioquest's ReadiUse™ NADP regenerating kit provides a flexible prepackaged NADP regenerating system to accommodate various experimental designs.
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Amplite® Colorimetric Lipase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric lipase activity assay kit offers a straightforward and efficient protocol for quantifying lipase activity.
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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.
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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.
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Amplite® Colorimetric Hexokinase Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Colorimetric Hexokinase Assay Kit is designed to accurately measure the activity of hexokinases from various sources using a simple and quick protocol.