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You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Amplite® Colorimetric Glucose-6-Phosphate Assay Kit

Amplite® Colorimetric Glucose-6-Phosphate Assay Kit

Supplier: AAT BIOQUEST

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

Supplier: AAT BIOQUEST

Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.

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Amplite® Colorimetric Enterokinase Activity Assay Kit

Amplite® Colorimetric Enterokinase Activity Assay Kit

Supplier: AAT BIOQUEST

Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.

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Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone.

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Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Lysyl oxidase (LOX) is an extracellular enzyme that catalyses formation of aldehydes from lysine residues in collagen and elastin precursors.

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Amplite® Colorimetric BCA Protein Quantitation Assay Kit

Amplite® Colorimetric BCA Protein Quantitation Assay Kit

Supplier: AAT BIOQUEST

The traditional BCA protein assay is widely used for quantifying proteins.

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Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Supplier: AAT BIOQUEST

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Amplite® Fluorimetric L-Lactate Assay Kit

Amplite® Fluorimetric L-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Serine hydrolase probes, ActivX™ FP

Serine hydrolase probes, ActivX™ FP

Supplier: Thermo Fisher Scientific

The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

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Amplite® Fluorimetric L-Alanine Assay Kit

Amplite® Fluorimetric L-Alanine Assay Kit

Supplier: AAT BIOQUEST

L-alanine (L-Ala) plays a crucial role as a building block of important proteins.

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Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*

Supplier: AAT BIOQUEST

Caspases play important roles in apoptosis and cell signaling.

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Amplite® Colorimetric Bradford Protein Quantitation Assay Kit

Amplite® Colorimetric Bradford Protein Quantitation Assay Kit

Supplier: AAT BIOQUEST

The traditional Bradford protein assay is widely used for quantifying protein concentrations.

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Amplite® Fluorimetric Sphingomyelin Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Sphingomyelin Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue.

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Amplite® Colorimetric Pyruvate Assay Kit

Amplite® Colorimetric Pyruvate Assay Kit

Supplier: AAT BIOQUEST

Pyruvate is an important chemical compound in intracellular metabolic pathways.

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Amplite® Fluorimetric Xanthine Assay Kit

Amplite® Fluorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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Amplite® Fluorimetric Lipase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity.

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Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Colorimetric NADPH Assay Kit

Amplite® Colorimetric NADPH Assay Kit

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric cADP-Ribose Assay Kit

Amplite® Fluorimetric cADP-Ribose Assay Kit

Supplier: AAT BIOQUEST

This cADP-ribose assay can be performed in a convenient 96-well or 384-well microtiter plate.

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Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit

Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit

Supplier: AAT BIOQUEST

Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.

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Amplite® Colorimetric Xanthine Assay Kit

Amplite® Colorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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Amplite® Colorimetric L-Alanine Assay Kit

Amplite® Colorimetric L-Alanine Assay Kit

Supplier: AAT BIOQUEST

L-alanine (L-Ala) plays a crucial role as a building block of important proteins.

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Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.

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Amplite® Fluorimetric D-Lactate Assay Kit

Amplite® Fluorimetric D-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Supplier: AAT BIOQUEST

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.

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Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit

Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit

Supplier: AAT BIOQUEST

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesised in liver and present in blood.

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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.

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Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

E. coli beta-galactosidase is a 464 Kd tetramer.

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Amplite® Colorimetric Aspartate Aminotransferase (AST) Assay Kit

Amplite® Colorimetric Aspartate Aminotransferase (AST) Assay Kit

Supplier: AAT BIOQUEST

Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.

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