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551 výsledky pro Cellular Assays

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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

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Oct4 transcription factor assay kit

Oct4 transcription factor assay kit

Supplier: CAYMAN

96-Well assay for measurement of Oct4.

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LYSO-ID® red cytotoxicity kit (GFP-CERTIFIED®) for microplates

Supplier: ENZO LIFE SCIENCES

A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay

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Hydrogen peroxide chemiluminescent detection Kit

Supplier: ENZO LIFE SCIENCES

For the quantitative determination of hydrogen peroxide in culture supernatants and cell lysates from any species.

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Caspase-3 assay kits, NucView™ 488

Caspase-3 assay kits, NucView™ 488

Supplier: BIOTIUM

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

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CV-Cathepsin B detection kit

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.

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Reagents for Fluor-de-Lys®-Green HDAC fluorometric activity assay kit

Supplier: ENZO LIFE SCIENCES

Cellular assay, Fluor-de-Lys®-green substrate, in 50 mM solution in DMSO

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CF® Dye Dextran 3500 MW, anionic and fixable

Supplier: BIOTIUM

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.

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CF® Dye Dextran 10000 MW, anionic and fixable

CF® Dye Dextran 10000 MW, anionic and fixable

Supplier: BIOTIUM

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.

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CF® Dye Dextran 70000 MW, anionic and fixable

CF® Dye Dextran 70000 MW, anionic and fixable

Supplier: BIOTIUM

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group.

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CF® Dye Dextran 250000 MW, anionic and fixable

CF® Dye Dextran 250000 MW, anionic and fixable

Supplier: BIOTIUM

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.

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Neutrophil/Monocyte Respiratory Burst Assay Kit

Neutrophil/Monocyte Respiratory Burst Assay Kit

Supplier: CAYMAN

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

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Mitochondria/cytosol fractionation kit

Supplier: ENZO LIFE SCIENCES

For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.

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Apoptosis and necrosis quantitation kits

Apoptosis and necrosis quantitation kits

Supplier: BIOTIUM

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

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Membrane potential detection kit, MITO-ID®

Membrane potential detection kit, MITO-ID®

Supplier: ENZO LIFE SCIENCES

MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.

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ROS-ID™ superoxide detection kit

Supplier: ENZO LIFE SCIENCES

This kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.

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Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells

Supplier: BIOTIUM

The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

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ViaFluor® 647 live cell microtubule stain 1000X in DMSO

Supplier: BIOTIUM

Stain microtubules in live cells with far-red fluorescence.

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Apoptosis and necrosis assay kit, NucView™488 RedDot™2

Supplier: BIOTIUM

This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

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p70 S6K activity kit

Supplier: ENZO LIFE SCIENCES

The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

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Cytochrome C releasing apoptosis assay kit

Supplier: ENZO LIFE SCIENCES

For the detection of Cytochrome c translocation from mitochondria into cytosol during apoptosis.

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LEADING LIGHT® Wnt cell line assay reagents

Supplier: ENZO LIFE SCIENCES

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

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NUCLEOLAR-ID® Green detection kit

Supplier: ENZO LIFE SCIENCES

Specifically designed for visualizing microscopically nucleoli in living cells.

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Hydrogen peroxide colorimetric detection kit

Supplier: ENZO LIFE SCIENCES

Hydrogen peroxide colorimetric detection kit provides a fast and higher throughput 96-well plate based homogeneous assay for the measurement of hydrogen peroxide in a wide variety of samples.

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FluoForte™ Reagent

Supplier: ENZO LIFE SCIENCES

Calcium dye

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CREB (Phospho-Ser133) transcription factor assay kit

Supplier: CAYMAN

A 96-well assay for measurement of CREB.

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TUNEL assay kits

TUNEL assay kits

Supplier: BIOTIUM

TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

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JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium

JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium

Supplier: BIOTIUM

JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.

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Reagents for Color-de-Lys™ HDAC colorimetric activity assay kit

Supplier: ENZO LIFE SCIENCES

Cellular assay, Color-de-Lys™ developer concentrate, 300 µl

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Lyso-ID® red detection kit (GFP-CERTIFIED®)

Lyso-ID® red detection kit (GFP-CERTIFIED®)

Supplier: ENZO LIFE SCIENCES

For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.

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Nitric Oxide (NO2-/NO3-) detection kit

Supplier: ENZO LIFE SCIENCES

For the quantitative determination of nitrite and nitrate levels in culture supernatants, plasma, serum, saliva, and urine from any species.

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