Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Buffer, HEPES sodium salt solution (1 mol/L), BioReagent, suitable for cell culture, Sigma-Aldrich®

Supplier: Merck

HEPES sodium salt buffer solution (1 mol/l)

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, TRIS solution pH 9,0 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 9,0 (1 mol/l)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).

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TISAB II Solution, Supelco®

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH meters.

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Standard reference materials and buffer solutions, CertiPUR®, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH instruments.

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Buffer solutions, according to EP and USP, Certipur®

Supplier: Merck

Buffer solutions intended for extremely precise calibration, qualification and monitoring of pH instruments, and for testing other buffer solutions.

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Dulbecco’s phosphate buffered saline (DPBS), Sigma-Aldrich®

Supplier: Merck

Dulbecco’s phosphate buffered saline is modified with/without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture.