Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology
Supplier: VWR Chemicals
Sodium acetate buffer, VWR®, 3 M, pH value: 5,2
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VWR® Phosphate Buffered Saline (PBS), High Phosphate
Supplier: VWR Chemicals
Irradiated solution, pH 7,2, for washing blots.
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GET buffer
Supplier: ROCKLAND
GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.
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Optimizer Buffer™ for optimal cross-linking and modification
Supplier: G BIOSCIENCES
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.
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RIPA cell lysis buffer 2
Supplier: Enzo Life Sciences
RIPA cell lysis buffer 2, Enzo Life Sciences
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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™
Supplier: G BIOSCIENCES
Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.
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VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade
Supplier: VWR Chemicals
PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.
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Fixation buffer for microscopy and flow cytometry
Supplier: BIOTIUM
Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.
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VWR® AVS TITRINORM, Cesium chloride/lanthanum chloride buffer solution acc. to schinkel for atomic absorption spectroscopy (aas)
Supplier: VWR Chemicals
This material is intended for use as an ionisation buffer for AAS
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Caspase cell lysis buffer
Supplier: Enzo Life Sciences
Caspase cell lysis buffer, Enzo Life Sciences
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Caspase assay buffer
Supplier: Enzo Life Sciences
50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.
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Buffer, PBS tablets
Supplier: PanReac AppliChem
PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)
Supplier: ROCKLAND
This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
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VWR® Sodium Acetate Buffer 3,0 M Solution, pH 5,2
Supplier: VWR Chemicals
With diethyl pyrocarbonate (DEPC).
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6X GelRed prestain loading buffer with tracking dye
Supplier: BIOTIUM
6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).
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VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology
Supplier: VWR Chemicals
Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)
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Buffers, potassium phosphate buffer (0,5 mol/L) solutions
Supplier: CAYMAN
Potassium phosphate buffer solution (0,5 mol/l), pH value: 9,0
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Wash buffer salts, lyophilised, Enzo Life Sciences
Supplier: Enzo Life Sciences
Wash Buffer Salts can be used in tissue preparations, immunohistochemical staining procedures and in assays that require HRP-linked detection reagents.
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VWR® AVS TITRINORM, Cesium chloride/aluminium nitrate buffer solution acc. to schuhknecht and schinkel for determination of na, k and li by flame aas
Supplier: VWR Chemicals
This material is intended for use as an ionisation buffer for AAS
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VWR® Phosphate Buffered Saline (PBS), Low Phosphate, 10X
Supplier: VWR Chemicals
Irradiated concentrate of PBS, suitable for use in washing cells after dilution.
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VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20
Supplier: VWR Chemicals
Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.
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Blocking buffer, fish gelatin
Supplier: BIOTIUM
Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
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Tris-Borate-EDTA (TBE) buffer, 10X
Supplier: PanReac AppliChem
10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4
Supplier: CAYMAN
Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).
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Competent cell buffer
Supplier: ROCKLAND
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.
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Blocking Buffer 10X
Supplier: ABCAM
Blocking Buffer 10X is part of the reagents, controls and accessories range.
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VWR®, Hybridisation buffers with 50% formamide
Supplier: VWR Chemicals
Hybridisation cocktail offering blocking and hybridisation for optimised complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridisation.
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Cell lysis buffer, RIPA
Supplier: Enzo Life Sciences
The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.
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Mini flip-top vial with 9 ml Butterfield's buffer
Supplier: 3M FOOD SAFETY
The 3M™ Mini Flip-Top Vial with 9 ml Butterfield's Buffer is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.