You searched for: Buffers

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

Supplier: GHM MESSTECHNIK

Cost-effective alternative: Buffer capsules and working sets. With buffer capsules, you can produce a 100 ml buffer solution in the most cost-effective manner. A particular advantage of the capsules is that their storage life is several years longer than that of ready-to-use solutions.

Supplier: MP BIOMEDICALS

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

Supplier: Thermo Fisher Scientific

This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Supplier: G BIOSCIENCES

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

Supplier: 3M FOOD SAFETY

The 3M™ Mini Flip-Top Vial with 9 ml Letheen Broth is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

Supplier: MP BIOMEDICALS

A peroxidase protective buffer which stabilises HRP conjugates. It has 500 ml of PBS, pH 7,2, containing 0,5% BSA, antimicrobial preservatives and proprietary stabilising compounds.

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Supplier: HONEYWELL

Matrix Modifier, 2% Potassium (K) in 1% HCl 1%

Supplier: Thermo Fisher Scientific

Sodium acetate, 3M aq, solution, pH 4,5, autoclaved

Supplier: Thermo Fisher Scientific

Sodium acetate, 3M aq, solution, pH 7,0, RNAse free, Liquid

Supplier: WTW

These standard buffer solutions (work reference buffer solutions) are widely used in precision applications.

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

Supplier: Thermo Fisher Scientific

Sodium acetate, 3M aq, solution, pH 7,0, autoclaved

Supplier: Thermo Fisher Scientific

Potassium dihydrogen phosphate and di-Sodium hydrogen phosphate in aqueous solution, Phosphate, pH value: 7,00, ±0,02

Supplier: Merck

Washing buffer, PBS buffer solution (phosphate buffered saline) pH 7,4

Supplier: Thermo Fisher Scientific

Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid

Supplier: Merck

Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)

Supplier: CPACHEM

Shelf Life on Ship Date: 12 months - Store under normal laboratory conditions, at temperatures between 15 to 25 °C.

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

Supplier: Merck

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Merck

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

Supplier: MP BIOMEDICALS

Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.