VWR®, dNTPs
Supplier: VWR Chemicals
Ready to use molecular biology grade dNTP mixes and dNTP sets. The dNTP mix is designed to save hands-on time for researchers, and reduce the possibility of contamination by reducing pipetting. dNTP solutions are also available in sets of four individual dNTPs, each 100 mm. Both are convenient for use in DNA polymerisation reactions, DNA labelling and sequencing processes.
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Peqlab peqGOLD, Deoxynucleotide Triphosphate (Solution)
Supplier: VWR Peqlab
Individually packaged deoxynucleotide triphosphates manufactured to the highest quality levels.
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dNTP mix
Supplier: BIOTIUM
Highly purified mix of dNTPs (>99% by HPLC) in 0,6 mM Tris-HCl (pH 7,5); each dNTP is at a concentration of 10 or 25 mm in the solution provided.
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5-Tetramethylrhodamine-dUTP, 1 mM Solution
Supplier: BIOTIUM
5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA to make fluorescently labeled probes, and has absorption/emission at 553/577 nm. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes. Supplied as a 1 mM solution in Tris-HCl buffer, pH 7.5.
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TriLink® N1-Methyl-Pseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
N1-Methyl-Pseudouridine-5'-Triphosphate is widely used in mRNA synthesis for therapeutic and vaccine development to improve mRNA functionality. This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.
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TriLink® 2'-O-Methyluridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® 2'-O-Methyluridine-5'-Triphosphate
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TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)
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TriLink® 5-Methylcytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.
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TriLink® Pseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.
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Cyanine 3-dUTP
Supplier: Enzo Life Sciences
Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.
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Cyanine 5-dUTP
Supplier: Enzo Life Sciences
Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.
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Cyanine 3-UTP (enhanced)
Supplier: Enzo Life Sciences
Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .
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Cyanine 5-UTP (enhanced)
Supplier: Enzo Life Sciences
Cyanine 5-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP.
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Fluorescein-12-ddUTP dideoxynucleotide pack for 3’-oligonucleotide labeling
Supplier: Enzo Life Sciences
The procedure for labeling of DNA probes with a polynucleotide 'tail' containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions.
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Bio-16-UTP
Supplier: Enzo Life Sciences
Bio-16-UTP (Biotin-16-uridine-5’-triphosphate) can replace UTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.
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Bio-17-ATP
Supplier: Enzo Life Sciences
Bio-17-ATP (Biotin-17-adenosine-5’-triphosphate) can replace ATP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.
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