Kovac's indole reagent for microbiology
Supplier: PanReac AppliChem
A reagent for the detection of indole-positive and indole-negative microorganisms. Some microorganisms can cleave tryptophan which is especially abundant in trypticalle digested peptone to give pyruvic acid, ammonia and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a colour reaction with 4-dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
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Kovac's indole reagent for microbiology pH <1 (H₂O, 20 °C), Millipore®
Supplier: Merck
Kovac's indole reagent for microbiology pH <1 (H₂O, 20 °C), Millipore®