Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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Succinate, 0,2 M buffer solution, pH 5,0

Supplier: Thermo Fisher Scientific

Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific

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MES lysis buffer with Triton® X-100

Supplier: Thermo Fisher Scientific

MES lysis buffer with Triton® X-100, Thermo Fisher Scientific

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Lysis buffers, red blood cells

Supplier: G BIOSCIENCES

These DNase- and RNase-free buffers and reagents are designed for high quality research.

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ACK Lysing Buffer (1x), TheraPEAK™

Supplier: LONZA

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.

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HEPES protein extraction buffer (5X)

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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NP-40 lysis buffer, low salt

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, low salt, Thermo Fisher Scientific

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MNE buffer, pH 6,5

Supplier: Thermo Fisher Scientific

MNE buffer, pH 6.5, Thermo Fisher Scientific

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GTE buffer

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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HEPES lysis buffer with NP-40 (2X)

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants

Supplier: G BIOSCIENCES

ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.

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RIPA Lysis and Extraction Buffer

Supplier: G BIOSCIENCES

A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.

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Protein extraction reagent, BugBuster® 10X, Millipore®

Supplier: NOVAGENE

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.