You searched for: Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
SLK Human shRNA Plasmid Kit (Locus ID 9748)
Supplier: ORIGENE TECHNOLOGIES
These shRNA constructs were designed against multiple splice variants at this gene locus.
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Cell-free protein expression vectors, T7, mammalian
Supplier: Thermo Fisher Scientific
The T7 cell-free expression vectors (pT7CFE1) are cloning plasmids optimised for use with the Thermo Scientific™ 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation.
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Transfection control plasmids, pVectOZ
Supplier: OZ BIOSCIENCES
pVectOZ transfection plasmids are engineered in an optimised backbone and are ideal for all transfections.
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Slc7a11 (NM_011990) mouse tagged ORF clone
Supplier: ORIGENE TECHNOLOGIES
Slc7a11 (GFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter y+ system) member 11 (Slc7a11), (10 µg).
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pET-24b(+) DNA, Novagen®
Supplier: NOVAGENE
The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).
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Expression vectors, pGEX
Supplier: Cytiva
The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.
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Expression vectors, green renilla Luc vector for luciferase assays, Pierce™
Supplier: Thermo Fisher Scientific
Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis
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Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors
Supplier: Thermo Fisher Scientific
Gaussia Dura Luc vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.
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M13KO7 Helper Phage, Cytiva
Supplier: Cytiva
M13 phage for the GST expression system.
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E. coli BL21
Supplier: Cytiva
The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
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Vector, pBR322, MP Biomedicals
Supplier: MP BIOMEDICALS
pBR322 is a 4363 bp vector purified from Escherichia coli. It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).
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Expression vectors, cypridina Luc vector for luciferase assays, Pierce™
Supplier: Thermo Fisher Scientific
Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca.
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Polyadenylic acid (poly-A)
Supplier: Cytiva
Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.
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Expression vectors, red firefly Luc vector for Luciferase assays, Pierce™
Supplier: Thermo Fisher Scientific
Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.
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Gaussia Luc vector for luciferase assays, expression vectors, Pierce™
Supplier: Thermo Fisher Scientific
Gaussia Luc vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.
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Vector, pUC 18, MP Biomedicals
Supplier: MP BIOMEDICALS
pUC 18 is a small vector of approximately 2,7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.
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PUC18 plasmid DNA
Supplier: Merck
Plasmids are isolated from host strain E. coli RRI. These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains.
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Polyadenylic acid (poly-A)
Supplier: Merck
Polyadenylic acid (poly-A) is used to evaluate binding on cationic liposomes doped with non-ionic nucleolipids. Poly-A is used in small molecule mRNA targeted drug development to evaluate the binding of potential therapeutic agents such as the isoquinoline group of alkaloids.
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Cell-free protein synthesis, rapid translation system (RTS) vectors
Supplier: biotechrabbit
Expression vectors for PCR-based generation of linear templates.