One-Step HIV-1 p24 Protein ELISA Kit
ORIGEA290002
New Product
:
- Pk:1 KIT
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Primary antibody reactivity:Virus
- Description:One-step HIV-1 p24 protein ELISA kit
- Sample type:Cell cuture supernatant, lentiviral sample
- Cross reactivity:There is no detectable cross-reactivity with other relevant proteins.
- Assay Principle:Quantitative
- Specificity:This kit is used for the detection of HIV-1 p24 protein
- Sample volume:1 µl of lentiviral sample or 90 µl diluted sample
- Sensitivity:4,18 pg/ml
The gag protein p24 (MW 24kD) is a major structural protein of the HIV capsid, which is required for virus particle assembly. There are approximately 2000 p24 molecules per virus particle, or at a molecule weight of 24 kDa, about 104 virus particles per picogram of p24.
- Components:
- HIV-1 p24 Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate
- Recombinant P24 Standard (100 ng/ml)|0,05 ml
- 1× P24 Detection Antibody-HRP Conjugate|2 ml
- Assay Buffer|60 ml
- Lysis Buffer 2 ml
- Wash Buffer Concentrate 20X|30 ml
- TMB Substrate|12 ml
- Stop Solution|12 ml
The onset of symptoms of AIDS correlates with an increased level of virus and p24 in the blood. HIV-1 p24 measurement is commonly used as an indicator of HIV-1 infection and viral load. In gene therapy, HIV-derived lentiviral vectors are commonly used to deliver transgenes to mammalian cells. Quantitative detection of the HIV-1 p24 has become a standard method for measuring lentiviral transduction efficiency.
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