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buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

 
 

Plasmid DNA buffers

Supplier: OMEGA BIO-TEK

Solution 1, resuspension buffer

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E.Z.N.A.® RNALock stabilizer reagent

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

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Immunoassay Blocking (BSA Free)

Immunoassay Blocking (BSA Free)

Supplier: Abcam

Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.

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RBC Lysis Buffer (10X)

RBC Lysis Buffer (10X)

Supplier: Tonbo Biosciences

RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.

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Electrophoresis running buffers

Electrophoresis running buffers

Supplier: G-Biosciences

There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.

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G-Biosciences Red Blood Cell Lysis Buffer

Supplier: G-Biosciences

A red blood cell lysis buffer for the isolation of white blood cells free from interference of RBCs. The optimised concentration of ammonium chloride and enhances effectively lyses red blood cells, while preserving the integrity of the leukocytes.

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Immunoassay Blocking Buffer

Immunoassay Blocking Buffer

Supplier: Abcam

Immunoassay blocking buffer effectively preserves the conformation and activity of dried proteins in immunoassays.

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10X RIPA Buffer, Abcam

Supplier: Abcam

10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells. - Cited in over 180 publications - Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot - Effectively extracts cytoplasmic, nuclear and membrane proteins

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Kinase Dilution Buffer III, Abcam

Supplier: Abcam

Solution of buffer used for diluting kinase protein.

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Kinase Assay Buffer I, Abcam

Supplier: Abcam

Stock solution of buffer used for assaying kinase protein.

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Extraction Buffer 5X PTR, Abcam

Supplier: Abcam

Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types

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Extraction Enhancer Buffer 50X, Abcam

Supplier: Abcam

Abcam's Extraction Enhancer Buffer 50X is formulated for the preparation of cell or tissues extracts for use in immunoassays.

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10X Wash Buffer PT

10X Wash Buffer PT

Supplier: Abcam

10X Wash Buffer PT (ab206977) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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20x PBS buffer with Tween 20, Abcam

Supplier: Abcam

20x Phosphate buffered saline (PBS) wash buffer with tween 20 (pH 6.5 - 6.9) for use during immunolabeling techniques such as immunohistochemistry (IHC). - Contains PBS, 1% sorbitan monolaurate, ethoxylated

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25x TBS (pH 7.4), Abcam

Supplier: Abcam

Tris Buffered Saline (TBS) wash buffer ab64248 for use during immunolabeling techniques such as IHC.

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Blocking Buffer 10X

Blocking Buffer 10X

Supplier: Abcam

Blocking Buffer 10X is part of the reagents, controls and accessories range.

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