buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Buffer, TRIS solution pH 8 (1 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/L)

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Buffers, sodium phosphate buffer solutions (0,5 mol/L)

Supplier: Cayman Chemical

Sodium phosphate stock solution (0,5 mol/L), pH value: 6,0

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Buffers, potassium phosphate buffer (0,5 mol/L) solutions

Supplier: Cayman Chemical

Potassium phosphate buffer solution (0,5 mol/l), pH value: 6,0

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Buffer, HEPES solution (1 mol/L)

Supplier: Cayman Chemical

HEPES buffer solution (1 mol/l)

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4

Supplier: Cayman Chemical

250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4

Supplier: Cayman Chemical

Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).

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10X Blocking Buffer, Abcam

Supplier: Abcam

10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative

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Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

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Cell Lysis Buffer, Abcam

Supplier: Abcam

ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.

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Native lysis Buffer, Abcam

Supplier: Abcam

Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.

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Fixation buffer for microscopy and flow cytometry

Supplier: Biotium

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

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Permeabilisation buffer

Supplier: Biotium

Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

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6X GelRed prestain loading buffer with tracking dye

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

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