Supplier: TriLink BioTechnologies

Cas9 Nickase mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.

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CleanScribe™ RNA Polymerase is a novel enzyme that reduces double-stranded RNA (dsRNA) formation in IVT RNA synthesis.

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Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.

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This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

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2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.

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TriLink® 5-Propargylamino-2'-deoxycytidine-5'-Triphosphate

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TriLink® 2'-O-Methylcytidine-5'-Triphosphate

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ChromaLink™ Didoxigenin contains a UV-traceable chromophore to enable reproducibility labelling. The degree of biotinylation can be measured in minutes, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan, biotin incorporation can be quantified and reproducible production of consistent batches ensured. ChromaLink™ labelling can be paired with NanoLink™ Streptavidin Magnetic Beads for many types of assay development.

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The Antibody-Oligonucleotide All-in-One Conjugation kit offers TurboLink™ catalysed conjugation based on Solulink’s proprietary linking technology to prepare antibody-oligonucleotide conjugates without chromatography. The kit includes buffers, spin columns, and a calculator to determine MSR. No column chromatography is required. With the Antibody-Oligonucleotide All-in-One Conjugation kit, you can generate high-purity conjugates virtually free of residual antibody or oligonucleotide (>95% conjugate).

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Fluorescein Antibody Conjugation kit offers an efficient method that delivers >95% conversion of antibody to conjugate. The kit includes everything needed to make two direct-labelled fluorescein-antibody conjugates from 100 µg of any user-supplied antibody.

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Biotin-16-Aminoallyl-2'-deoxyuridine can be enzymatically incorporated into DNA via PCR, nick translation, primer extension using Klenow(exo-) DNA polymerase, and terminal deoxynucleotidyl transferase (TdT). Biotinylated cDNA can be prepared with this NTP using Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.

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TriLink® N1-Methyl-2'-O-Methylpseudouridine-5'-Triphosphate

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This construct expresses a firefly luciferase (FLuc) protein, originally isolated from the firefly Photinus pyralis, as self-amplifying RNA (saRNA). FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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TriLink® Cytidine-5'-O-(1-Thiotriphosphate)

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TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)

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TriLink® 2'-Deoxycytidine-5'-O-(1-Thiotriphosphate)

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2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability.

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TriLink® 2'-O-Methyl-N6-Methyladenosine-5'-Triphosphate

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2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

Supplier: TriLink BioTechnologies

This construct expresses an enhanced version of the green fluorescent protein (EGFP), originally isolated from the jellyfish Aequorea victoria, as self-amplifying RNA (saRNA). EGFP is a commonly used direct detection reporter in mammalian cells, yielding bright green fluorescence with an emission peak at 509 nm.

Supplier: TriLink BioTechnologies

ChromaLink™ Biotin Protein Labelling kit offers a complete solution for the quantitative biotinylation of proteins from 20 kDa to 200 kDa and from 25 µg to 1 mg. The kit incorporates traceable biotin on proteins and allows for direct spectrophotometric verification of biotin incorporation. A simple Å280 and Å354 measurement determines the number of biotins per protein molecule.

Supplier: TriLink BioTechnologies

5-Propargylamino-2'-deoxyuridine can be enzymatically incorporated into DNA and cDNA to introduce primary amine groups. Primary amines can be labeled with NHS ester fluors and haptens for detection and immobilisation of the resulting DNA strand.

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TriLink® 7-Deaza-7-Propargylamino-2'-deoxyguanosine-5'-Triphosphate

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This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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Cyanine 5-Aminoallyluridine-5'-Triphosphate is a substrate for T7 RNA polymerase, allowing the incorporation of cyanine 5 into RNA and mRNA during in vitro transcription. Cyanine 5 incorporation allows for fluorescent detection of RNA and mRNA independent of protein translation.

Supplier: TriLink BioTechnologies

The Protein-Protein Conjugation kit is designed to easily and efficiently conjugate two proteins together. This kit is flexible so that researchers with little or no conjugation experience can make their own custom protein-protein conjugates to suit their needs. It includes all of the necessary components - including S-HyNic and S-4FB - and protocols for easy and specific crosslinking of any protein with any other protein of a different size greater than 7 kDa.

Supplier: TriLink BioTechnologies

β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.