"Stemcell Technologies"

Supplier: Stemcell Technologies

Hamster (Armenian) monoclonal antibody against mouse CD80 (B7-1), PE-conjugated.

Supplier: Stemcell Technologies

Mouse monoclonal IgM, kappa isotype control antibody, PE-conjugated.

Supplier: Stemcell Technologies

Immunomagnetic positive selection of mouse cells labeled with APC-conjugated antibodies.

Supplier: Stemcell Technologies

Mouse monoclonal IgG2b antibody against human CD68, Alexa Fluor® 488-conjugated.

Supplier: Stemcell Technologies

Tumor necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine that activates NF-κB, MAPK, and PI3K/AKT pathways. Activated T cells and macrophages are the primary producers of TNF-α in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, among them B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α has cytotoxic effects on cancerous cells by stimulating anti-tumor immunosuppressive responses. TNF-α stimulates expression of E- and P-selectins, thus facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová and Hošek). Other effects of TNF-α include vasodilatation and edema formation. In vitro studies of adult rat neural progenitor cells (NPCs) demonstrate that TNF-α reduces neurogenesis in dentate gyrus-derived NPCs, and promotes astrogliogenesis in subventricular zone-derived NPCs (Borsini et al.).

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched human total lymphocytes

Supplier: Stemcell Technologies

Differentiation and maturation kits for generation of motor neurons from human pluripotent stem cells.

Supplier: Stemcell Technologies

Rat monoclonal IgG2b antibody against mouse CD117 (c-Kit), biotin-conjugated.

Supplier: Stemcell Technologies

Rat monoclonal IgM antibody against mouse CD43, biotin-conjugated.

Supplier: Stemcell Technologies

Platelet-derived growth factor (PDGF) is a dimeric glycoprotein consisting of two disulfide bridge-stabilized polypeptide chains, A and B, which are assembled as heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) (Fretto et al.; Westermark and Heldin). PDGF signals through the receptor tyrosine kinases PDGFRalpha and PDGFRbeta. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin- like fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). It has been shown that PDGF-AB together with 5-Azacytidine (Catalog #72012), induces the conversion of mature bone and fat cells into tissue-regenerative multipotent stem cells (Chandrakanthan et al.).

Supplier: Stemcell Technologies

Methylcellulose-based medium with agar-LCM (without EPO) for human cells.

Supplier: Stemcell Technologies

Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.

Supplier: Stemcell Technologies

Fast and easy isolation of extracellular vesicles using immunomagnetic positive selection.

Supplier: Stemcell Technologies

For culture and evaluation of human mammary epithelial cells in CFU assays.

Supplier: Stemcell Technologies

Epidermal growth factor (EGF) is the founding member of the EGF family of proteins and is characterized by high affinity binding to various EGF receptors (EGFRs) and the production of mitogenic responses (Carpenter and Cohen). EGF promotes EGFR dimerization, resulting in activation of downstream pathways including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling. EGF is secreted by the gut-associated salivary and Brunner’s glands, is found in a variety of body fluids, and stimulates cell proliferation and differentiation in rodent and neonatal human intestine (Wright et al.). EGF can stimulate the proliferation of mouse embryonic stem cells or induce the terminal differentiation/growth inhibition of A431 cells (Heo et al.).

Supplier: Stemcell Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a, FITC-conjugated.