1,3-Diphenylacetone-p-toluenesulphonylhydrazone ≥98%, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
1,3-Diphenylacetone-p-toluenesulphonylhydrazone ≥98%, Sigma-Aldrich®
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Rhodamine 123 fluorescent dye, mitochondria dye
Supplier: SIGMA ALDRICH MICROSCOPY
Rhodamine 123 is useful as a laser dye and to have selective cell growth effects. It has also been used to measure mitochondrial membrane potential.
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Silver Protein
Supplier: SIGMA ALDRICH MICROSCOPY
Protargol silver impregnation and neuronal selectivity for light and electron microscopic observations.
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Haematoxylin solution Gill III, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time. Used with hematoxylin and eosin staining.
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Giemsa's stain modified, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
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Amyloid Stain, Congo Red Kit, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Amyloid protein is detected in tissue with Congo red, a metachromatic stain. Staining is intensified by pretreatment of tissue with alkaline sodium chloride.
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Papanicolaou's solution EA 65, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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Formalin 10% histological fixative, neutral buffered, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Formalin solution (10%, neutral buffered) is a widely used fixative for samples processed for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.
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Reticulum Stain Kit acc. to Gordon & Sweets, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
The kit is based upon the method of Gordon and Sweets.
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Tartrazine, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Yellow counterstain suitable for a wide range of histological staining procedures.
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Silver methenamine
Supplier: SIGMA ALDRICH MICROSCOPY
Intended for use in the silver stain (modified GMS) procedure.
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Haematoxylin modified solution (Harris), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
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Giemsa's stain, certified by the Biological Stain Commission, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections. Giemsa is a versatile polychromatic stain, which is suitable for staining a diverse range of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites. It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa. The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. Giemsa′s staining solution is composed of methylene blue, azure, and eosin.
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bisbenzimide H-33342 trihydrochloride (Hoechst 33342) ≥98% (by HPLC, TLC), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
bisBenzimide H 33342 trihydrochloride has been used for the staining of viable and apoptotic.
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Wright's stain modified, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Popular hematology stain used for differentially staining the cellular elements of blood. For dip, rack, and batch staining techniques.
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Rhodamine 6G ~95% (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Rhodamine 6G has been used as a leukocyte marker to study the interaction between leukocytes and the vascular endothelium. It has also been used to label platelets.
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Reticulocyte stain, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Utilizes New Methylene Blue for the identification of reticulocytes in blood films.
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Sudan III, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Sudan III, Sigma-Aldrich®
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Methyl green zinc chloride salt, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Methyl green zinc chloride salt, Sigma-Aldrich®
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Acridine orange base ≥75% (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.
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Pyronin G, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Pyronin G, Sigma-Aldrich®
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Fast Red Violet LB Salt ≥90% (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fast Red Violet LB Salt has been used for the detection of tartrate-resistant acid phosphatase (TRAP) in tissue sections.
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Fluorescent brightener 28, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps.
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Bromocresol purple sodium salt, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Bromocresol purple sodium salt, Sigma-Aldrich®
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Percoll®, Siam
Supplier: SIGMA ALDRICH MICROSCOPY
Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15 – 30 nm diameter (23% w/w in water), coated with polyvinylpyrrolidone (PVP).
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Solvent Blue 38, Practical grade, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Solvent Blue 38 is a phthalocyanine stain, used for staining myelin, neurons and glial fibres.
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Direct yellow 27, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
As a direct dye, Direct Yellow 27 is an acid dye with large molecules that will bind to cellulose fibers directly. It is mono azo dye with good light fastness.
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4,7-Dihydroxy-1,10-phenanthroline ≥30% (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
4,7-Dihydroxy-1,10-phenanthroline, Dye content ≥30 % (1,10-Phenanthroline-4,7-diol)
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Methyl violet 2B for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Methyl violet for microscopy (Bact., Bot., Hist.), indicator (pH 0.1 - 2.0), mixture of polymethylated pararosaniline hydrochlorides.
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Carmine, powder, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Carmine is a red compound and is generally used with aluminum, iron and other metal salts to enhance its activity. During smear preparation, it is required for chromatin staining in fresh material. In presence of aluminum chloride, it is used for the staining of glycogen.