2498 Results for: "PCR"

Supplier: Quantabio

AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

Supplier: IKA

Shaker, Vortex Mixer, PCR plate attachment, 96-well

Supplier: Quantabio

Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ Taq DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified Taq DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.

Supplier: AGILENT

Polyadenylation of miRNA from total RNA and synthesis of first-strand cDNA from tailed miRNAs.

Supplier: VWR Collection

Pre-cut aluminum foil seals on a roll for PCR applications. Supplied on a roll with holder for ease of use.

Supplier: Biotium

Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq Gold by having a faster activation time and better shelf-life.

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

Supplier: VWR Collection

Solid blocks: For use as a low temperature hotplate or for custom drilling.

Supplier: Thermo Fisher Scientific

Clear polyester film, aluminium foil. Recommended for use in PCR in conjunction with a thermal cycler that has a screw-down or clip-down lid.

Supplier: VWR Collection

This robust cooler is consistent and reliable. It is made from engineered PP with a PC lid. The modern design cools faster in freezers and stays cold longer on the bench top.

Supplier: Thermo Fisher Scientific

Clear, 24-well format Piko® PCR/qPCR film seal allows high transmission of fluorescence across the range of commonly used fluorophores. When used with a Piko® thermal cycler or PikoReal® Real-Time PCR system, sample volumes as low as 5 µl can be used with minimal evaporative loss.

Supplier: ENZO LIFE SCIENCES

Ultra-pure, stable, and suitable for a variety of PCR applications.

Supplier: AGILENT

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.

Supplier: AGILENT

The StrataClone PCR cloning kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

Supplier: GOLD STANDARD DIAGNOSTICS

This RT-PCR assay is a highly sensitive Multiplex Real-Time PCR with CE-IVD marking for the direct qualitative pathogen detection of SARS-CoV-2 genomic RNA in respiratory samples.

Supplier: ENZO LIFE SCIENCES

AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: GRANT INSTRUMENTS

Advanced benchtop UV cabinets providing aseptic conditions for a variety of biomedical and biochemical procedures. Innovative dual UV system: built-in UV-air recirculator provides constant decontamination of the air volume within the cabinet while working and traditional surface UV decontamination while the door is closed.

Supplier: Thermo Scientific

Increase nucleic acid sample separation by using the Thermo Scientific™ 10×5 ml rotor with ClickSeal™ biocontainment lid.

Supplier: Bel-Art Products, a Part of SP

These storage boxes are designed to fit in a standard 2" freezer racks. This 144-place PCR tube freezer box will hold individual and/or strip 0,2 ml PCR tubes.

Supplier: VWR Chemicals

VWR® DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

Supplier: IKA

Block heater block, Double block, For: 96-well PCR plate (1), Depth: 15,5 mm

Supplier: Thermo Fisher Scientific

Automated nucleic acid purification system, accessory, Thermo Scientific™ KingFisher™ Apex 96 PCR head

Supplier: THERMO MATRIX TECHNOLOGIES

Heat sealer, accessory, Plate carrier for non-skirted PCR plates, ALPS™

Supplier: GOLD STANDARD DIAGNOSTICS

This Real-Time RT-PCR assay is intended for the qualitative detection of SARS-CoV-2 from human nasopharyngeal swabs, eluted directly into deionised water without an additional RNA extraction process.

Supplier: AGILENT

Herculase II Fusion DNA Polymerases produce superior yields with extension times as short as 15 s/kb, even with genomic DNA targets up to 23 kb.

Supplier: Quantabio

PerfeCTa® MultiPlex qPCR ToughMix® is a 5X concentrated, ready to use reaction cocktail for Real-Time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix® allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta® MultiPlex qPCR ToughMix® has been optimised to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.

Supplier: GOLD STANDARD DIAGNOSTICS

Real-Time RT-PCR kit for the qualitative detection and discrimination of relevant mutations associated with SARS-CoV-2 variants: B.1.1.7 (UK), B.1.351 (South Africa), and B.1.1.28 (P.1) (Brazil).

Supplier: AGILENT

Brilliant II SYBR® QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: AGILENT

Brilliant III Ultra-Fast SYBR® QPCR and QRT-PCR Master Mixes deliver superior specificity by utilizing a novel faster-activating hot start method to minimize the formation of primer-dimers and other off-target reactions.