16885 Results for: "Enzo Life Sciences"

Supplier: Enzo Life Sciences

Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye.

Supplier: Enzo Life Sciences

Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.

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Anti-GRP78 Rabbit Polyclonal Antibody

Supplier: Enzo Life Sciences

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

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Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. KLK8 is one of the fifteen kallikrein subfamily member. The KLK8 enzyme exhibits distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.

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This protein belongs to the serine protease family. The encoded protein contains a serine protease domain, a transmembrane domain, an LDL receptor-like domain, and a scavenger receptor cysteine-rich domain. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to human diseases and disorders. This protein was identified by its association with both congenital and childhood onset autosomal recessive deafness. This protein is expressed in fetal cochlea and many other tissues, and is thought to be involved in the development and maintenance of the inner ear or the contents of the perilymph and endolymph. This protein was also identified as a tumor-associated gene that is overexpressed in ovarian tumors. Alternatively spliced transcript variants have been described.

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Anti-HSP90B1 Rabbit Polyclonal Antibody

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Produced in E. coli.

Supplier: Enzo Life Sciences

Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.

Supplier: Enzo Life Sciences

Liquid extract in a SDS-PAGE sample buffer (pH: 6,8) containing 40 mM TRIS-HCl, 1% SDS, 50 mM DTT, 7,5% glycerol and 0,003% bromophenol blue.

Supplier: Enzo Life Sciences

The conversion of arachidonic acid to prostaglandin H2 (PGH2) by cyclooxygenase (COX) enzymes is the first step in the synthesis of prostanoids. Prostaglandin D2 (PGD2) is synthesized from PGH2 by the action of PGD synthase, and its actions in vasodilation and platelet activation are mediated via activation of two identified G-protein coupled receptors (GPCRs): DP1 and DP2 (formerly CRTH2). Both DP1 and DP2 receptors are coupled to Gs alpha subunits, resulting in increased adenylyl cyclase activity and cAMP production upon agonist binding. DP1 receptor is expressed moderately in the ileum and weakly in the lung, stomach, and uterus of mice, and weakly in the small intestine of humans. In rodents, expression of PGD synthase and the DP1 receptor is seen in the leptomeninges, where it is believed they play a role in the induction of sleep. The DP1 receptor may also oppose DP2-mediated activation of basophils, eosinophils, and Th2 cells.

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Endothelins are vasoactive peptides that exist in three forms (ET-1, ET-2, and ET-3), and function in the maintenance of vascular tone via two identified seven transmembrane-spanning domain-containing G protein-coupled receptors (GPCR), endothelin A (ETA) and endothelin B (ETB). The human ETA receptor is primarily expressed in vascular smooth muscle cells, including those of the heart (also in myocytes), lung, and brain, and its stimulation generally results in vasoconstriction. Activation of ETA receptors can be stimulated by binding of ET-1 and ET-2, but not ET-3. triggering the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells. Human ETB receptors are primarily expressed in endothelial cells lining the vessel walls of the lungs, heart, and brain, and in contrast to ETA receptors, stimulation generally results in transient vasodilation. Activation of ETB receptors can be stimulated by binding of all three endothelins, triggering the release of vasorelaxive

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Synapse-associated proteins SAP97 and SAP102 display extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminals. In addition to the CNS, SAP97 is also detected at the basal lateral membrane between a variety of epithelial cells. SAP102 associates with NMDA receptors through the C-terminal NR2B subunit of the receptor. The N-terminal SAP97 PDZ domain mediates its interaction with the synaptic ras-GTPase activating protein SynGAP.

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Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Seven subclasses (5-HT 1-7) of serotonin receptors have been identified, a majority of which are members of the seven transmembrane domain-containing G-protein coupled receptor (GPCR) family, and are the target of several anti-depressant drugs. The 5-HT1 subclass consists of five highly homologous receptors that couple via G(i/o) alpha subunits to inhibit adenylyl cyclase activity. The 5-HT1A receptor gene is 99% homologous between human and mouse, and is expressed from an intronless gene with a predicted length of 421 amino acids. 5-HT1A receptor activation is generally inhibitory to cAMP production, but may also stimulate type II adenylyl cyclase activity in a cell-specific manner via release of G(beta/gamma) subunits when coupled to G(i2). 5-HT1A expression in mammals is strongest in CNS limbic pathways that control emotion, including tissue of the hippocampus, dorsal raphé, and septum.

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The actions of the octapeptide circulatory hormone angiotensin II are mediated by type one (AT1) and type two (AT2) angiotensin II receptors, members of the seven-transmembrane domain-containing GPCR family. AT1 expression is greatest in vascular smooth muscle, liver, kidney, adrenal cortex, brain, and lung, and is primarily responsible for the physiological effects of angiotensin II in cardiovascular homeostasis. The AT2 receptor displays approximately 30% identity to the AT1 receptor, and is highly expressed during embryonic development. AT2 expression in adult tissue is limited to the adrenal medulla, brain, and reproductive tissues, but is upregulated in injured tissue following myocardial infarction, vascular injury, and wound healing.

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The opioid receptors δ, κ, and μ are members of the seven-transmembrane domain-containing receptor family of G-protein coupled receptors. δ- and μ-receptors serve as functional receptors for endogenous enkephalin and β-endorphin peptide ligands, respectively. δ-opioid receptors are highly expressed in the olfactory bulb, neocortex, caudate putamen and nucleus accumbens, with peripheral expression in the gastro-intestinal tract and vas deferens. δ-receptors inhibit neurotransmitter release resulting in a variety of biological effects including analgesia, motor integration, gastro-intestinal motility, olfaction, respiration, and memory. Neural μ-receptor expression is prominent in the caudate putamen, neocortex, thalamus, nucleus accumbens, hippocampus, and amygdala, as well as in peripheral nervous tissues in the gut. Named for its pharmacological mediation of analgesia by morphine, the μ receptor typically functions as a depressant in physiological processes including respiration, cardiovascular function, gastrointestinal motility, and thermoregulation.

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Adrenergic receptors mediate the hormone and neurotransmitter functions of adrenaline and noradrenaline. Adrenoreceptors function in peripheral tissues and the central nervous system to regulate numerous vascular, metabolic, and neurological functions, and are the target for many therapeutically relevant drugs including those for the treatment of hypertension, asthma, benign prostatic hypertrophy, and depression. Adrenoreceptors are classified by three types and multiple subclasses (alpha1A,1B,1D; alpha2A,2B,2C; and beta1,2,3) based on pharmacological and molecular characteristics.

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Produced in E. coli. E. coli thioredoxin is fused at the N-terminus to a His-tag.

Supplier: Enzo Life Sciences

Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA and Bax, and that the signaling pathways regulated by these genes together promote a full cell death response.

Supplier: Enzo Life Sciences

Synapse-associated proteins SAP97 and SAP102 display extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminals. In addition to the CNS, SAP97 is also detected at the basal lateral membrane between a variety of epithelial cells. SAP102 associates with NMDA receptors through the C-terminal NR2B subunit of the receptor. The N-terminal SAP97 PDZ domain mediates its interaction with the synaptic ras-GTPase activating protein SynGAP.

Supplier: Enzo Life Sciences

The TLRs (Toll-like receptors) are a group of evolutionarily conserved pattern recognition molecules that play a key role in host defense during microbial infection by regulating both innate and adaptive immune responses. TLRs are type I transmembrane proteins with ectodomains containing interspersed leucine-rich repeat (LRR) motifs that are involved in the recognition of various microbial ligands.

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The Jurkat T lymphocyte cell line was established from the peripheral blood of a 14-year old boy with T cell leukaemia.

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Produced in E. coli.

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Potent cell permeable and metabolically stable specific inhibitor of hSIRT1 (IC50=98nM in vivo / IC50=38nM in vitro; compared to hSIRT2: IC50=19µM and hSIRT3: IC50=48µM) with no effect on human histone deacetylases (HDACs) class I and class II, nor NAD glycohydrolase (IC50>100µg). Inhibits the deacetylation of p53 (IC50=1µM).

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Produced in HEK 293 cells. The extracellular domain of human netrin-1 (aa 28-604) is fused at the N-terminus to a FLAG®-tag.

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Sterile and endotoxin-free PBS for use with TLRgrade™ or endotoxin-free grade reagents.

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Host: Mouse, Isotype: IgG1

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The PATHO-GENE® HPV type 6/11 probe is a mixture of biotin-labeled HPV 6 and HPV 11-specific probes in buffered formamide and hybridisation enhancers.

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The slide contains two wells of fixed CaSki cells with HPV 16 genomic DNA and is used as a positive control for DNA in situ hybridisation of HPV-16.

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CGH labelling kit for BAC arrays has been optimised to label DNA for dual sample comparative genomic hybridisation (CGH) assays on BAC arrays. It is suitable for the preparation of cyanine 3- and cyanine 5-labelled DNA for hybridisation to BAC arrays.