"Biowest"

Supplier: Biowest

L(+)-Glutamine 0.2 mol/l in aqueous solution

Supplier: Biowest

Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

Supplier: Biowest

Amphotericin B used in tissue culture to prevent growth of yeasts and fungi. It changes the function and integrity of eucaryotic cell membranes by forming complexes with sterols (cholesterol) hence having no effect on bacteria. Cause leakage of glucose.

Supplier: Biowest

Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Supplier: Biowest

Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

Supplier: Biowest

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

Supplier: Biowest

Gentamycin sulphate acts against Gram-negative, Gram-positive bacteria and mycoplasma.

Supplier: Biowest

Lymphosep is a standardised, high quality medium designed for simple and rapid isolation of PBMCs from treated whole blood. Lymphosep, like Ficoll™, has the optimised density 1,077 g/ml for lymphocyte separation to guarantee best and reliable results for your preparations.

Supplier: Biowest

Dulbecco's Modified Eagle Medium (DMEM) is a modification of Basal Medium Eagle (BME) which contains approximately four times as much of the vitamins and amino acids, and two to four times as much glucose (depending on the version) as the original formulation.

Supplier: Biowest

The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Supplier: Biowest

L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

Supplier: Biowest

Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

Supplier: Biowest

Penicillin-Streptomycin acts against Gram-negative and Gram-positive bacteria.

Supplier: Biowest

Newborn Calf Serum (NBCS) originates from 3 to 10 day old calves. It is a high quality, cost effective replacement for Foetal Bovine Serum (FBS) and contains a somewhat higher proportion of proteins and immunoglobulin, but fewer growth factors. With proper cell adaptation, it has been shown to perform very well in cell culture applications where routine cell growth and cell culture maintenance is the objective.

Supplier: Biowest

Human serum 'off-the-clot' is processed from human blood that has been coagulation-induced. It is collected from volunteer donors.

Supplier: Biowest

Plasma provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth. It is used as a component of bioassays, immunoassays or enzyme assays.