"Biotium"
dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range
Supplier: Biotium
AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.
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Cell scrapers
Supplier: Biotium
PE, disposable, sterile. Mini cell scrapers for harvesting cells or cell lysates from microwell plates.
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qPCR master mixes, Forget-Me-Not™ EvaGreen®
Supplier: Biotium
EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.
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Wheat germ agglutinin (WGA) conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™
Supplier: Biotium
EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.
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One-Step Blue® protein gel stain
Supplier: Biotium
One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing.
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Nucleic acid stains, PAGE GelRed™
Supplier: Biotium
Stains specifically designed for DNA in polyacrylamide gels.
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GelRed® & GelGreen® Nucleic acid gel stains
Supplier: Biotium
GelRed and GelGreen are highly sensitive DNA/RNA gel stains made safe by preventing penetration into cells.
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EvaGreen® Plus dye, 20X in Water
Supplier: Biotium
EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.
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SYBR® Green I 10000x in DMSO nucleic acid gel stain
Supplier: Biotium
Thiazole green, which is structurally identical to SYBR® green I nucleic acid gel stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.
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PMAxx™ dye 20 mM in water
Supplier: Biotium
PMAxx™ dye is a DNA modifier used for viability PCR (v-PCR). V-PCR is a powerful PCR-based, culture-independent, method for rapid and sensitive quantification of microbial viability.
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Propidium iodide 1 mg/ml in water
Supplier: Biotium
Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.
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Decontamination bags, activated charcoal
Supplier: Biotium
Activated charcoal retains ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.
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PMA (Propidium monoazide) DNA/RNA binding dye
Supplier: Biotium
PMA (propidium monoazide) is a high-affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids.
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Fluorometric polymerase activity assay kit, EvaEZ™
Supplier: Biotium
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
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Cell viability assay kits
Supplier: Biotium
MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.



