This construct expresses a firefly luciferase (FLuc) protein, originally isolated from the firefly Photinus pyralis, as self-amplifying RNA (saRNA). FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

• Due to their self-amplification capability, saRNAs may provide more durable and longer protein expression than mRNAs. This ready-to-use saRNA construct is:

• Capped using CleanCap® Reagent AU, mimicking the authentic 5′ alphavirus end while providing an optimal Cap-1 structure with >95% capping efficiency, for efficient expression in mammalian cells.
• Synthesized using CleanScribe™ RNA Polymerase, reducing unwanted double-stranded RNA (dsRNA) by-products by up to 85%, for lower inflammatory responses and enhanced functionality.
• Manufactured using TriLink’s proprietary CleanScript® saRNA in vitro transcription (IVT) method, improving saRNA yield and integrity while also lowering dsRNA formation, for enhanced protein expression.
• Unmodifid or modified with 5-methylcytidine (5mC), which can increase nuclease stability, reduce interaction of innate immune receptors, and enhance translational efficiency of saRNAs.

Our catalog saRNA construct employs alphaviral replication machinery from Venezuelan equine encephalitis virus (VEEV) that encodes for the non-structural proteins (nsP1-4). It is polyadenylated (26A) and optimized for mammalian systems.

Concentration: 1 mg/ml