EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit

Catalog # STMC17865

Supplier: STEMCELL Technologies

Catalog # STMC17865

Supplier: STEMCELL Technologies

Specifications

Description:
EasySep™ human central and effector memory CD4+ T cell isolation kit, 1×10⁹ cells
Environmentally Preferable:
Pk:
1 KIT

Specifications

About this item

Immunomagnetic positive selection of human central and effector memory CD4+ T cells.

Easy-to-use and column-free
Up to 96% purity
No-wash removal of EasySep™ Releasable RapidSpheres™

This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.

Caution: Product Restrictions: (i) Customer shall not make products or any portion of them, in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier's mark of origin without express written permission of Supplier; and (ii) Customer is familiar with Supplier's restrictions on the use of the Products and shall use the Products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').