About this item
Column-free immunomagnetic positive selection of human B cells for HLA analysis.
- Fast and easy-to-use
- Up to 97% purity
- No columns required
The EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit is designed to isolate B cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognising CD19, CD20 and dextran-coated magnetic particles. labelled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors, but is lost upon maturation to plasma cells. The antigen CD20 is expressed on all stages of B cell development except on pro B cells or plasma cells.
This product replaces the EasySep™ HLA Whole Blood B Cell Positive Selection Kit for even faster cell isolations.